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| SILVA,VICTOR D.A.; CUEVAS,CARLOS; MUÑOZ,PATRICIA; VILLA,MONICA; AHUMADA-CASTRO,ULISES; HUENCHUGUALA,SANDRO; SANTOS,CLEONICE C. DOS; ARAUJO,FILLIPE M. DE; FERREIRA,RAFAEL S; SILVA,VANESSA B. DA; SILVA,JULIANA H.C. E; SOARES,ÉRICA N.; VELOZO,EUDES S.; SEGURA-AGUILAR,JUAN; COSTA,SILVIA L.. |
| ABSTRACT Prosopis juliflora is a shrub that has been used to feed animals and humans. However, a synergistic action of piperidine alkaloids has been suggested to be responsible for neurotoxic damage observed in animals. We investigated the involvement of programmed cell death (PCD) and autophagy on the mechanism of cell death induced by a total extract (TAE) of alkaloids and fraction (F32) from P. juliflora leaves composed majoritary of juliprosopine in a model of neuron/glial cell co-culture. We saw that TAE (30 µg/mL) and F32 (7.5 µg/mL) induced reduction in ATP levels and changes in mitochondrial membrane potential at 12 h exposure. Moreover, TAE and F32 induced caspase-9 activation, nuclear condensation and neuronal death at 16 h exposure. After 4 h,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Programmed cell death; Autophagy; Glial cells; Neurons; Piperidine alkaloids; Prosopis juliflora.. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000100247 |
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| NUÑEZ,MARCO T; GARATE,MARCO A; ARREDONDO,MIGUEL; TAPlA,VICTORIA; MUÑOZ,PATRICIA. |
| Cells tightly regulate iron levels through the activity of iron regulatory proteins (IRPs) that bind to RNA motifs called iron responsive elements (IREs). When cells become iron-depleted, IRPs bind to IREs present in the mRNAs of ferritin and the transferrin receptor, resulting in diminished translation of the ferritin mRNA and increased translation of the transferrin receptor mRNA. Similarly, body iron homeostasis is maintained through the control of intestinal iron absorption. Intestinal epithelia cells sense body iron through the basolateral endocytosis of plasma transferrin. Transterrin endocytosis results in enterocytes whose iron content will depend on the iron saturation of plasma transferrin. Cell iron levels, in turn, inversely correlate with... |
| Tipo: Journal article |
Palavras-chave: Metal ions; Intestinal absorption; Ferritin; Transferrin; Homeostasis. |
| Ano: 2000 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602000000200013 |
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