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| Engels,Cibelle; Fuganti-Pagliarini,Renata; Marin,Silvana Regina Rockenbach; Marcelino-Guimarães,Francismar Corrêa; Oliveira,Maria Cristina Neves; Kanamori,Norihito; Mizoi,Junya; Nakashima,Kazuo; Yamaguchi-Shinozaki,Kazuko; Nepomuceno,Alexandre Lima. |
| The loss of soybean yield to Brazilian producers because of a water deficit in the 2011-2012 season was 12.9%. To reduce such losses, molecular biology techniques, including plant transformation, can be used to insert genes of interest into conventional soybean cultivars to produce lines that are more tolerant to drought. The abscisic acid (ABA)-independent Dehydration Responsive Element Binding (DREB) gene family has been used to obtain plants with increased tolerance to abiotic stresses. In the present study, the rd29A:AtDREB2A CA gene from Arabidopsis thaliana was inserted into soybean using biolistics. Seventy-eight genetically modified (GM) soybean lines containing 2-17 copies of the AtDREB2A CA gene were produced. Two GM soybean lines (P1397 and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Arabidopsis; Differential expression; Genetically modified organism; Water deficit. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000400015 |
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| Almeida,Hebréia Oliveira; Barbosa,Meire de Oliveira; Marques,Ana Ermelinda; Pereira,Tânus Henrique Abdalla; Magalhães Júnior,Marcos Jorge; Tessarollo,Nayara Gusmão; Games,Patrícia Dias; Barros,Everaldo Gonçalves de; Stolf-Moreira,Renata; Marcelino-Guimarães,Francismar Corrêa; Abdelnoor,Ricardo Vilela; Pereira,Paulo Roberto Gomes; Baracat-Pereira,Maria Cristina. |
| O objetivo deste trabalho foi avaliar, por meio de enzimas marcadoras, a indução de resistência à ferrugem-asiática-da-soja em genótipos de soja contrastantes quanto à suscetibilidade a Phakopsora pachyrhizi. Aproteína total e as atividades de cinco enzimas marcadoras da indução de resistência (lipoxigenases, peroxidases, fenilalanina amônia-liase, quitinases e β-1, 3-glucanases) foram avaliadas em extratos de folhas de plantas de soja dos genótipos Embrapa 48 (suscetível) e PI 561356 (resistente), submetidas à inoculação ou não com o patógeno. Foram observadas respostas de defesa discrepantes entre os dois genótipos e entre os tempos de coleta (12, 72 e 168 horas após inoculação). A resposta de indução dessas enzimas assemelha-se à defesa bifásica, para... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Glycine max; Phakopsora pachyrhizi; Atividade enzimática; Mecanismos de defesa; Resistência sistêmica; Resposta em cascata. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2012000200003 |
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| Stolf-Moreira,Renata; Lemos,Eliana Gertrudes de Macedo; Abdelnoor,Ricardo Vilela; Beneventi,Magda Aparecida; Rolla,Amanda Alves Paiva; Pereira,Selma dos Santos; Oliveira,Maria Cristina Neves de; Nepomuceno,Alexandre Lima; Marcelino-Guimarães,Francismar Corrêa. |
| The objective of this work was to validate, by quantitative PCR in real time (RT-qPCR), genes to be used as reference in studies of gene expression in soybean in drought-stressed trials. Four genes commonly used in soybean were evaluated: Gmβ-actin, GmGAPDH, GmLectin and GmRNAr18S. Total RNA was extracted from six samples: three from roots in a hydroponic system with different drought intensities (0, 25, 50, 75 and 100 minutes of water stress), and three from leaves of plants grown in sand with different soil moistures (15, 5 and 2.5% gravimetric humidity). The raw cycle threshold (Ct) data were analyzed, and the efficiency of each primer was calculated for an overall analysis of the Ct range among the different samples. The GeNorm application was used to... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Glycine max; Expression stability; RT-qPCR; Water deficit. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2011000100008 |
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