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Santos,Karina Freire D'eça Nogueira; Silveira,Ricardo Diógenes Dias; Martin-Didonet,Claudia Cristina Garcia; Brondani,Claudio. |
- The objective of this work was to determine the total protein profile and the contents of the four major protein fractions (albumin, globulin, prolamin and glutelin) and of the amino acids in the endosperm of the rice wild species Oryza glumaepatula. The experiment was performed with 29 accessions of this species, collected from 13 Brazilian locations, and two commercial cultivars. Protein samples were prepared using dried, polished, and ground grains to obtain homogeneous, dry flour used in the preparation of extracts. Oryza glumaepatula accessions were identified with the highest levels of total protein, albumin and glutelin protein fractions, and amino acids (with the exception of tryptophan) in comparison to the two analized rice cultivars. The... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Oryza sativa; Genetic resources; Nutritional quality; Plant breeding; SDS-Page. |
Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2013000100009 |
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Ferreira,Enderson Petrônio de Brito; Stone,Luis Fernando; Martin-Didonet,Claudia Cristina Garcia. |
RESUMO Os atributos da microbiota do solo são importantes indicadores de alterações causadas por práticas agrícolas. Foi conduzido um experimento em condição de campo, em um delineamento de blocos casualizados com quatro repetições, com o objetivo de avaliar os efeitos da forma de manejo (sistema de semeadura direta - SSD e sistema de plantio convencional - SPC) e da fase de condução da cultura do arroz de terras altas (pré-plantio - PP, floração - FA e pós-colheita - PC) sobre atributos da biomassa microbiana do solo (Cmic, Nmic, RBS, qCO2), atividade enzimática (AET, Aβ-G e AFA) e população de microrganismos (bactérias, fungos, actinobactérias e diazotróficos) em amostras de solo coletadas na profundidade de 0-20 cm, tendo como referência uma área de... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Oryza sativa; Manejo do solo; Qualidade do solo; Contagem de microrganismos. |
Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902017000100022 |
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Cardoso,Aline Assis; Andraus,Michel de Paula; Borba,Tereza Cristina de Oliveira; Martin-Didonet,Claudia Cristina Garcia; Ferreira,Enderson Petrônio de Brito. |
Abstract This study aimed to evaluate the tolerance to salinity and temperature, the genetic diversity and the symbiotic efficiency of rhizobia isolates obtained from wild genotypes of common bean cultivated in soil samples from the States of Goiás, Minas Gerais and Paraná. The isolates were subjected to different NaCl concentrations (0%, 1%, 2%, 4% and 6%) at different temperatures (28 °C, 33 °C, 38 °C, 43 °C and 48 °C). Genotypic characterization was performed based on BOX-PCR, REP-PCR markers and 16S rRNA sequencing. An evaluation of symbiotic efficiency was carried out under greenhouse conditions in autoclaved Leonard jars. Among 98 isolates about 45% of them and Rhizobium freirei PRF81 showed a high tolerance to temperature, while 24 isolates and... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Phaseolus vulgaris; Rhizobium; Symbiotic efficiency; Carbon source use; Tolerance to salinity and temperature. |
Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000100043 |
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Braga,Lívia Fabiana; Oliveira,Fênix Araújo de; Couto,Eva Aparecida Prado do; Santos,Karina Freire d'Eça Nogueira; Ferreira,Enderson Petrônio de Brito; Martin-Didonet,Claudia Cristina Garcia. |
ABSTRACT This work aimed to characterize 20 isolates obtained from upland rice plants, based on phenotypic (morphology, enzymatic activity, inorganic phosphate solubilization, carbon source use, antagonism), genotypic assays (16S rRNA sequencing) and plant growth promotion. Results showed a great morphological, metabolic and genetic variability among bacterial isolates. All isolates showed positive activity for catalase and protease enzymes and, 90% of the isolates showed positive activity for amylase, catalase and, nitrogenase. All isolates were able to metabolize sucrose and malic acid in contrast with mannitol, which was metabolized only by one isolate. For the other carbon sources, we observed a great variability in its use by the isolates. Most... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Oryza sativa; PGPR; Associative bacteria; Bacterial diversity. |
Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000100020 |
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