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Dual transcriptomics of virus-host interactions: comparing two Pacific oyster families presenting contrasted susceptibility to ostreid herpesvirus 1 ArchiMer
Segarra, Amelie; Mauduit, Florian; Faury, Nicole; Trancart, Suzanne; Degremont, Lionel; Tourbiez, Delphine; Haffner, Philippe; Barbosa-solomieu, Valerie; Pepin, Jean-francois; Travers, Marie-agnes; Renault, Tristan.
Background: Massive mortality outbreaks affecting Pacific oyster (Crassostrea gigas) spat in various countries have been associated with the detection of a herpesvirus called ostreid herpesvirus type 1 (OsHV-1). However, few studies have been performed to understand and follow viral gene expression, as it has been done in vertebrate herpesviruses. In this work, experimental infection trials of C. gigas spat with OsHV-1 were conducted in order to test the susceptibility of several bi-parental oyster families to this virus and to analyze host-pathogen interactions using in vivo transcriptomic approaches. Results: The divergent response of these oyster families in terms of mortality confirmed that susceptibility to OsHV-1 infection has a significant...
Tipo: Text Palavras-chave: Interactions; Ostreid herpesvirus 1; Gene expression; Susceptibility; Crassostrea gigas.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00199/31029/29431.pdf
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Influence of crude oil exposure on cardiac function and thermal tolerance of juvenile rainbow trout and European sea bass ArchiMer
Anttila, Katja; Mauduit, Florian; Le Floch, Stephane; Claireaux, Guy; Nikinmaa, Mikko.
Oil spills pose a threat to aquatic organisms. However, the physiological effects of crude oil on cardiac function and on thermal tolerance of juvenile fish are still poorly understood. Consequently, in this paper, we will present results of two separate experiments where we exposed juvenile rainbow trout and European sea bass to crude oil and made cardiac thermal tolerances and maximum heart rate (fHmax) measurements after 1 week (rainbow trout) and 6-month recovery (sea bass). In both species, the fHmax was lower in crude oil-exposed fish than in the control ones at temperatures below the optimum but this difference disappeared at higher temperatures. More importantly, the oil-exposed fish had significantly higher Arrhenius break point temperature for...
Tipo: Text Palavras-chave: Arrhenius break point temperature; Critical thermalmaximum; CTMAX; Fish; Heart rate; Oil spill; PAH.
Ano: 2017 URL: https://archimer.ifremer.fr/doc/00391/50271/50970.pdf
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Assessing chronic fish health: An application to a case of an acute exposure to chemically treated crude oil ArchiMer
Mauduit, Florian; Domenici, P.; Farrell, A. P.; Lacroix, C.; Le Floch, S.; Lemaire, P.; Nicolas-kopec, A.; Whittington, M.; Zambonino-infante, Jose-luis; Claireaux, Guy.
Human alteration of marine ecosystems is substantial and growing. Yet, no adequate methodology exists that provides reliable predictions of how environmental degradation will affect these ecosystems at a relevant level of biological organization. The primary objective of this study was to develop a methodology to evaluate a fish’s capacity to face a well-established environmental challenge, an exposure to chemically dispersed oil, and characterize the long-term consequences. Therefore, we applied high-throughput, non-lethal challenge tests to assess hypoxia tolerance, temperature susceptibility and maximal swimming speed as proxies for a fish’s functional integrity. These whole animal challenge tests were implemented before (1 month) and after (1 month)...
Tipo: Text Palavras-chave: Fish; Oil spill; Dispersant; Biomarkers; Physiological integrity; Ecological performance; Temperature; Hypoxia; Swimming; Growth.
Ano: 2016 URL: https://archimer.ifremer.fr/doc/00347/45828/45916.pdf
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Domestication compromises athleticism and respiratory plasticity in response to aerobic exercise training in Atlantic salmon (Salmo salar) ArchiMer
Zhang, Yangfan; Timmerhaus, Gerrit; Anttila, Katja; Mauduit, Florian; Jorgensen, Sven Martin; Kristensen, Torstein; Claireaux, Guy; Takle, Harald; Farrell, Anthony P..
Commercially selective breeding of Atlantic salmon (Salmo salar) primarily for rapid growth may compromise cardiorespiratory robustness and its related phenotypes. Therefore, a suite of respiratory indices was used to evaluate aerobic capacity and hypoxia tolerance to test the hypothesis that exercise training can improve the athletic robustness in both domesticated and wild strains of Atlantic salmon, but with the domesticated strain having a less cardiorespiratory plasticity and a lower athletic robustness than the wild strain. We also tested a second hypothesis that a constant acceleration screening protocol should segregate fish according to athletic robustness based on their swimming ability. These hypotheses were tested with parr from Bolaks...
Tipo: Text Palavras-chave: Athletic robustness; Atlantic salmon; Domestication; Exercise training; Hypoxia tolerance; Respiratory performance.
Ano: 2016 URL: https://archimer.ifremer.fr/doc/00334/44518/44214.pdf
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Assessing the long-term effect of exposure to dispersant-treated oil on fish health using hypoxia tolerance and temperature susceptibility as ecologically relevant biomarkers ArchiMer
Mauduit, Florian; Farrell, Anthony P.; Domenici, Paolo; Lacroix, Camille; Le Floch, Stephane; Lemaire, Pphilippe; Nicolas-kopec, Annabelle; Whittington, Mark; Le Bayon, Nicolas; Zambonino-infante, Jose-luis; Claireaux, Guy.
Consequences of exposure to polyaromatic hydrocarbons (PAH), toxic components of crude oil, on fish has been widely documented due to their ecological and economical importance. However, although forming a valuable and consistent body of knowledge, use of these data in spill response is limited. Objective of the present study was thus to facilitate the translation of published data into information of operational value. For this, we investigated the dose‐response relationship between dispersant‐treated oil exposure and ecologically‐relevant consequences by combining laboratory and field experiments. Effects were examined over almost a year using juveniles of the slow growing, commercially important European sea bass (Dicentrarchus labrax). Moreover, a...
Tipo: Text Palavras-chave: Oil spills; Polycyclic aromatic hydrocarbons (PAHs); Fish indices; Hypoxia; Temperature.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00455/56679/58438.pdf
Registros recuperados: 5
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