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Efeito do fósforo na captação de minerais e pigmentação de Azolla caroliniana Willd. (Azollaceae) 88
Adalberto,Paulo R.; Massabni,Antonio C.; Goulart,Antonio J.; Monti,Rubens; Lacava,Pedro M..
O bioacúmulo de minerais, a fixação de nitrogênio e a concentração de pigmentos em Azolla caroliniana foram estabelecidos sob estresse nutritivo. As culturas foram mantidas em frascos de poliestireno, utilizando gradiente de fosfato entre 10-7 e 10-3 mol.L-1. Os minerais (P, S, B e Sn) foram quantificados por espectrometria de emissão ótica com plasma de acoplamento induzido e o teor de nitrogênio pelo método de Kjeldahl. A atividade da nitrogenase foi determinada pela reação de redução do acetileno e as concentrações de pigmentos foram determinadas por fotometria nos extratos metanólicos acidificados. A absorção de macronutrientes (N, P, S) mostrou-se relacionada ao suprimento de fósforo, enquanto a absorção de micronutrientes (B e Sn) não sofreu este...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antocianina; Azolla caroliniana; Bioacumulação de minerais; Nitrogenase.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84042004000300017
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Isolation of natural inhibitors of papain obtained from Carica papaya latex 52
Monti,Rubens; Contiero,Jonas; Goulart,Antonio José.
Studies were carried out to natural papain inhibitor from papaya latex. Fresh latex from green fruits of Carica papaya was collected and immediately transported in ice bath to the lab, from which three fractions with inhibitor effect of esterase papain activity were isolated by latex dialysis, Sephadex G-25 gel filtration and ionic exchange chromatography in SP-Sephadex C-25. The isolated fractions, identified as inhibitors I and II, showed a negative reaction with ninhydrin; however, the fraction identified as P-III showed positive reaction with ninhydrin. Kinetics data showed non-competitive inhibition (inhibitor I) and uncompetitive (inhibitors II and P-III).
Tipo: Info:eu-repo/semantics/article Palavras-chave: Esterase activity; Natural papain inhibitor; Protease inhibitor.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000500010
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Microbial rennet produced by Mucor miehei in solid-state and submerged fermentation 52
Silveira,Guilherme Garcia da; Oliveira,Gustavo Monteiro de; Ribeiro,Eloizio Julio; Monti,Rubens; Contiero,Jonas.
The aim of this work was to study the effect of carbon and nitrogen sources in submerged fermentation, and the casein effect on solid-state fermentation on rennin production by Mucor miehei. Biomass peaks reached 6.7; 8.1 and 8 g/L and enzymatic production peaks of 1,066; 857 and 480 Soxhlet Units (S.U.) for glucose concentration of 18; 25 and 35 g/L respectively. Flasks with baffles showed biomass peaks of 6.7; 8.3 and 10 g/L and enzyme activity peaks of 648; 279 and 300 S.U. for the same glucose concentration. The values of 923 and 667 S.U. were obtained when corn steep liquor and Proflo (Cottonseed Nutrients from Traders ®) were used. In SSF system the enzymatic activities were 414, 264 and 167 S.U., when using HCl 0.2 N, 0.3 N and 0.4 N respectively....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mucor miehei; Rennin; Clotting activity; Casein; Solid-state fermentation; Submerged fermentation.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000800009
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Partial purification and properties of cellulase-free alkaline xylanase produced by Rhizopus stolonifer in solid-state fermentation 52
Goulart,Antonio José; Carmona,Eleonora Cano; Monti,Rubens.
Rhizopus stolonifer was cultivated in wheat bran to produce a cellulase-free alkaline xylanase. The purified enzyme obtained after molecular exclusion chromatography in Sephacryl S-200 HR showed optimum temperature as 45º C and hydrolysis pHs optima as pH 6.0 and 9.0. Xylanase presented higher Vmax at pH 9.0 (0.87 µmol/mg protein) than at pH 6.0 and minor Km at pH 6.0 (7.42 mg/mL) than at pH 9.0.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Alkaline xylanase; Rhizopus stolonifer; Solid-state fermentation.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000300001
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Effects of culture conditions on the production of inulinase by Kluyveromyces marxianus 52
Cazetta,Marcia Luciana; Monti,Rubens; Contiero,Jonas.
The present study was conducted to investigate the influence of initial sucrose concentration, pH and aeration rate on biomass and inulinase production by Kluyveromyces marxianus var. bulgaricus in a stirred batch reactor. Maximum inulinase activity (15.29 UmL-1) was obtained at a sucrose concentration of 10 g L-1, pH 5.0 and aeration rate of 1 vvm. The 20 g L-1 sucrose concentration was suitable for cell growth; however, enzymatic activity at this concentration was inhibited due to catabolic repression. The increase in aeration rate caused a reduction in enzyme activity with no relevant biomass increase.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Kluyveromyces marxianus var. bulgaricus; Sucrose; Inulinase; Biomass; Batch fermentation.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000300027
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Purification of papain from fresh latex of Carica papaya 52
Monti,Rubens; Basilio,Carmelita A.; Trevisan,Henrique C.; Contiero,Jonas.
In the present study we wish to report a method of crystallizing papain from fresh papaya latex which gave higher yields than previously reported. This method does not involve the use of sulphydryl reagents. The papain thus obtained is practically pure and shows a single band when submitted to electrophoresis on polyacrylamide gel, and is identical to the papain obtained by other methods. In routine enzymatic assays, specific activity was measured using Z-gly-pNP and BAEE as substrates. Papain crystallized by this method, without the use of high concentrations of salts or thiol-containing substances such as cysteine and dithiothreitol, is obtained in the form of a complex with natural inhibitors existent in latex which can be removed by dialysis.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Papain; Carica papaya; Cysteine proteinase; Proteolytic enzymes; Purification of papain.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000500009
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Cyclodextrin glycosyltransferase production by new Bacillus sp. strains isolated from brazilian soil 58
Menocci,Vivian; Goulart,Antonio José; Adalberto,Paulo Roberto; Tavano,Olga Luisa; Marques,Daniela Parreira; Contiero,Jonas; Monti,Rubens.
Three strains of Bacillus sp. (BACRP, BACNC-1 and BACAR) were isolated from soil adhered to cassava husk. CGTase specific activity for the three isolated strains was higher when cultivated at 40ºC. Potato starch, cassava starch, maltodextrin and glucose were used as carbon source and growth temperatures varied from 25 to 55ºC. The three isolates presented higher CGTase specific activity when cultivated with potato starch at 40ºC. Isolated BACRP and BACAR presented specific activity of 4.0x10-3 and 2.2x10-3 U/mg prot at pH 7.0, respectively, when cultivated in mediums added with NaCl 2%; at pH 10,0 their activities were of 3.4x10-3 and 3.0x10-3 U/mg prot, respectively, in the same concentration of NaCl. On the other hand, the isolated BACNC-1 presented...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cyclodextrin glycosyltransferase; CGTase production.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000400016
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Production and purification of an Endo–1,4-beta-Xylanase from Humicola grisea var. thermoidea by electroelution 58
Monti,Rubens; Cardello,Leonardo; Custódio,Marcos F.; Goulart,Antonio J.; Sayama,Adriana H.; Contiero,Jonas.
Humicola grisea var. thermoidea produces two forms of extracellular xylanase. The component form 1 was purified using the electroelution method, due to the very small production of this extracellular enzyme. The apparent molecular mass was 61.8 kDa by SDS-PAGE.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Electroelution; Enzyme purification; Extracellular xylanase; Humicola grisea var. thermoidea.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000200007
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Cyclodextrin glycosyltransferase from Bacillus licheniformis: optimization of production and its properties 58
Bonilha,Paulo Roberto Martins; Menocci,Vivian; Goulart,Antonio José; Polizeli,Maria de Lourdes Teixeira de Moraes; Monti,Rubens.
Cyclodextrin glycosyltransferase (EC 2.4.1.19) is an enzyme that produces cyclodextrins from starch via an intramolecular transglycosylation reaction. An alkalophilic Bacillus strain, isolated from cassava peels, was identified as Bacillus licheniformis. CGTase production by this strain was better when potato starch was used as carbon source, followed by cassava starch and amylopectin. Glucose and amylose, on the other hand, acted as synthesis repressors. When the cultivation was supplemented with sodium ions and had the pH adjusted between 6.0 and 9.0, the microorganism maintained the growth and enzyme production capacity. This data is interesting because it contradicts the concept that alkalophilic microorganisms do not grow in this pH range. After...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cyclodextrin glycosyltransferase; CGTase production; Bacilluslicheniformis; Bacillus beta-CD.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000300022
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Production of CGTase by a Bacillus alkalophilic CGII strain isolated from wastewater of a manioc flour industry 58
Freitas,Telma Luisa de; Monti,Rubens; Contiero,Jonas.
GCTase production by a new strain of Bacillus alkalophilic CGII isolated from Brazilian wastewater of manioc flour industry was examined. The growth medium used was composed by 1.5% starch, 1.5% nitrogen and 1% Na2CO3. Higher activity was obtained with starch, maltodextrin and galactose. When glucose was added to the medium, no enzyme production was observed. High enzyme activity and growth were reached when aeration was increased (88.6 U/mL). The enzyme characterization showed an optimum pH and temperature 8.0 and 55ºC for starch hydrolyses, respectively. Mg+ and Ca++ showed small activation; however, Hg+ and Cu+ showed a strong enzyme inhibition.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacillus alkalophilic CGII; CGTase; Beta-cyclodextrin; Starch hydrolysis; Enzyme activity.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000200015
Registros recuperados: 10
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