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Effect of temperature shock on soybean microspore embryogenesis BABT
Moraes,Ana Paula de; Bonadese-Zanettini,Maria Helena; Callegari-Jacques,Sídia Maria; Kaltchuk-Santos,Eliane.
In this study the effect of cold and heat shock on androgenetic induction in soybean anther culture was tested. Anthers of soybean were subjected to 4 and 33ºC, while the anthers used for control were maintained at 25ºC. Cytological analysis done during the 30 days of culture showed that frequencies of symmetrical binucleated and multicellular pollen grain did not differ among temperature treatments. Multicellular pollen grain could be formed by symmetrical division, as well as by assymetrical division. In relation to the embryo induction, the results of the treatments did not differ too. These results suggested that these treatments did not induce a sporophytic pathway in soybean.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anther culture; Soybean; Androgenesis; Symmetrical mitosis; Temperature shock; Stress.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000400006
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Morphological markers for microspore developmental stage in maize BABT
Moraes,Ana Paula de; Bered,Fernanda; Carvalho,Fernando Irajá Felix de; Kaltchuk-Santos,Eliane.
The use of maize in anther culture has been limited because only few genotypes presented a high androgenetic potential. Obtaining the proper stage of microspore development at culture initiation is one of the most crucial factors for success in the androgenesis. For Brazilian maize genotypes there are no studies reporting a correlation between cytological features and morphological parameters. In this study, morphological parameters were recorded and associated with cytological specific stages of the the microsporogenesis in two Brazilian maize genotypes that were sowed in different places (field and growing chamber). For both genotypes, the plants of the growing chamber presented a delay in development. Spikelets length and anther length/spikelet length...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Androgenesis; Anther culture; Microsporogenesis; Zea mays.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000500006
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