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Naveed,Muhammad; Ahmed,Iftikhar; Khalid,Nauman; Mumtaz,Abdul Samad. |
Glucose dehydrogenase (GDH; EC 1.1. 5.2) is the member of quinoproteins group that use the redox cofactor pyrroloquinoline quinoine, calcium ions and glucose as substrate for its activity. In present study, Leclercia sp. QAU-66, isolated from rhizosphere of Vigna mungo, was characterized for phosphate solubilization and the role of GDH in plant growth promotion of Phaseolus vulgaris. The strain QAU-66 had ability to solubilize phosphorus and significantly (p < 0.05) promoted the shoot and root lengths of Phaseolus vulgaris. The structural determination of GDH protein was carried out using bioinformatics tools like Pfam, InterProScan, I-TASSER and COFACTOR. These tools predicted the structural based functional homology of pyrroloquinoline quinone domains... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Glucose dehydrogenase; Pyrroloquinoline quinone; Leclercia sp; PGPR; Bioinformatics. |
Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200031 |
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Naveed,Muhammad; Mubeen,Samavia; khan,SamiUllah; Ahmed,Iftikhar; Khalid,Nauman; Suleria,Hafiz Ansar Rasul; Bano,Asghari; Mumtaz,Abdul Samad. |
In the present study, samples of rhizosphere and root nodules were collected from different areas of Pakistan to isolate plant growth promoting rhizobacteria. Identification of bacterial isolates was made by 16S rRNA gene sequence analysis and taxonomical confirmation on EzTaxon Server. The identified bacterial strains were belonged to 5 genera i.e. Ensifer, Bacillus, Pseudomona, Leclercia and Rhizobium. Phylogenetic analysis inferred from 16S rRNA gene sequences showed the evolutionary relationship of bacterial strains with the respective genera. Based on phylogenetic analysis, some candidate novel species were also identified. The bacterial strains were also characterized for morphological, physiological, biochemical tests and glucose dehydrogenase (gdh)... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Rhizosphere; Root nodules; 16S rRNA gene sequence; Glucose dehydrogenase; Pyrroloquinolone quinone. |
Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300031 |
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