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Carmo,Cátia Dias do; Silva,Maiane Suzarte da; Oliveira,Gilmara Alvarenga Fachardo; Oliveira,Eder Jorge de. |
The geminivirus complex known as cassava mosaic disease (CMD) is one of the most devastating viruses for cassava (Manihot esculenta Crantz). The aim of this study was to use molecular-assisted selection (MAS) to identify CMD-resistant accessions and ascertain promising crosses with elite Brazilian varieties. One thousand two hundred twenty-four accessions were genotyped using five molecular markers (NS169, NS158, SSRY028, SSRY040 and RME1) that were associated with resistance to CMD, along with 402 SNPs (single-nucleotide polymorphism). The promising crosses were identified using a discriminant analysis of main component (DAPC), and the matrix of genomic relationship was estimated with SNP markers. The CMD1 gene, previously described in M. glaziovii, was... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Manihot esculenta; Breeding; Disease; Virus. |
Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162015000600520 |
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Oliveira,Gilmara Alvarenga Fachardo; Pádua,Juliano Gomes; Costa,Juliana Leles; Jesus,Onildo Nunes de; Carvalho,Fabiana Moraes de; Oliveira,Eder Jorge de. |
The aim of this study was to evaluate the selected 41 SSR markers developed for yellow passion fruit (Passiflora edulis f. flavicarpa Sims.) for their transferability to 11 different Passiflora species. Twenty-one SSR were successfully amplified in 10 wild species of passion fruit producing 101 bands. All the markers were amplifiable for at least one species. The mean transferability was 68,8%, ranging from 15,4% (primer PE11) to 100 % (PE13, PE18, PE37, PE41 and PE88). Transferability was higher for the species from the Passiflora subgenus than for those from the Decaloba and Dysosmia subgenus. The results indicated a high level of nucleotide sequence conservation of the primer regions in the species evaluated, and consequently, they could potentially be... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Transferability; SSR; Passion fruit. |
Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000500009 |
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