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	Provedor de dados BABT (1) Autor Alegre,Ranulfo Monte (1) Padilha,Giovana da Silva (1) Santana,José Carlos Curvelo (1) Tambourgi,Elias Basile (1) Palavra-chave Aqueous two phase system (1) Burkholderia cepacia (1) Characterization (1) Lipase (1) Partitioning (1) Tipo do documento Info:eu-repo/semantics/article (1) Ano 2012 (1) País Brazil (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última Extraction of lipase from Burkholderia cepacia by PEG/Phosphate ATPS and its biochemical characterization BABT Padilha,Giovana da Silva; Santana,José Carlos Curvelo; Alegre,Ranulfo Monte; Tambourgi,Elias Basile. This work aimed to study the partitioning of a lipase produced by Burkholderia cepacia in PEG/Phosphate aqueous two phase system (ATPS) and its characterization. Lipase was produced by B. cepacia strains in a fermenter. Enzyme partitioning occurred at pH 6.0 and 8.0, using PEG 1500 and 6000 on two tie lines. Metal ions, pH and temperature effects on enzyme activity were evaluated. Five milliliter of 7.5% olive oil emulsion with 2.5% gumarabic in 0.1M sodium phosphate buffer at pH 8.0 and 37ºC were used for the activity determinations. Results showed that crude stratum from B. cepacia was partitioned by PEG1500/phosphate ATPS at pH 6.0 or 8.0 for, which the partitioning coefficients were 108-and 209-folds. Lipase presented optimal activity conditions at... Tipo: Info:eu-repo/semantics/article Palavras-chave: Lipase; Burkholderia cepacia; Aqueous two phase system; Partitioning; Characterization. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000100002 Registros recuperados: 1 Primeira ... 1 ... Última

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