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	Provedor de dados ArchiMer (1) Autor Brayton, P (1) Gimes, D (1) Huq, S (1) Palmer, L (1) Roszak, D (1) Palavra-chave Bacteria (1) Bacterial counters (1) Experimental research (1) Fluorescence microscopy (1) Marine environment (1) Microbial contamination (1) Pathogenic bacteria (1) Pollution detection (1) Vibrio cholerae (1) Mais... Tipo do documento Text (1) Ano 1984 (1) País France (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última Fluorescent antibody enumeration of Vibrio cholerae in the marine environment. ArchiMer Brayton, P; Roszak, D; Palmer, L; Huq, S; Gimes, D. From microcosm studies, V. cholerae and related potential human pathogens enter a viable but nonculturable state. Direct viable counts by epifluorescent microscopy consistently remain higher than corresponding plate counts. Thus, the assumption that pathogens "die-off" or "decay" in the marine environment must be re-evaluated, since stressed or nutrient-starved cells are unable to grow and be enumerated by standard plate count methods. Indirect immunofluorescent microscopy offers a more sensitive detection system for environment sampling for human pathogens. Field indicate that cultures positive for V. cholerae O1 are also positive by fluorescent antibody staining; however, the reverse was not necessarily true. By adapting epifluorescent techniques for... Tipo: Text Palavras-chave: Vibrio cholerae; Bacteria; Experimental research; Marine environment; Pathogenic bacteria; Microbial contamination; Fluorescence microscopy; Bacterial counters; Pollution detection. Ano: 1984 URL: http://archimer.ifremer.fr/doc/1984/acte-1000.pdf Registros recuperados: 1 Primeira ... 1 ... Última

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