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	Provedor de dados Biocell (1) Electron. J. Biotechnol. (1) Autor Paniego,Norma (2) Talia,Paola (2) Greizerstein,Eduardo J. (1) Heinz,Ruth A. (1) Heinz,Ruth Amelia (1) Hopp,H. Esteban (1) Hopp,Horacio Esteban (1) Nishinakamasu,Verónica (1) Poggio,Lidia (1) Mais... Palavra-chave Sunflower (2) BAC-FISH (1) C-PRINS (1) EST-SSR (1) InDels (1) Linkage map (1) Physical mapping (1) SSR (1) Single-copy sequences (1) Mais... Tipo do documento Info:eu-repo/semantics/article (1) Journal article (1) Ano 2011 (1) 2010 (1) País Argentina (1) Chile (1) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Genetic mapping of EST-SSR, SSR and InDel to improve saturation of genomic regions in a previously developed sunflower map Electron. J. Biotechnol. Talia,Paola; Nishinakamasu,Verónica; Hopp,Horacio Esteban; Heinz,Ruth Amelia; Paniego,Norma. In order to saturate a sunflower genetic map and facilitate marker-assisted selection (MAS) breeding for stress response, it is necessary to enhance map saturation with molecular markers localized in linkage groups associated to genomic regions involved in these traits. This work describes the identification and characterization of 1,134 simple sequence repeat (SSR) containing expressed sequence tags (EST) from unigenes available databases. Twelve of these functional markers as well as 41 public SSR markers were successfully localized in linkage groups, thus contributing to the saturation of specific regions on a reference genetic-linkage-map derived from recombinant inbred lines (RIL) mapping population from the cross between PAC2 x RHA266 lines. The... Tipo: Journal article Palavras-chave: EST-SSR; InDels; Linkage map; SSR; Sunflower. Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000600007 Detection of single copy sequences using BAC-FISH and C-PRINS techniques in sunflower chromosomes Biocell Talia,Paola; Greizerstein,Eduardo J.; Hopp,H. Esteban; Paniego,Norma; Poggio,Lidia; Heinz,Ruth A.. Bacterial artificial chromosome - fluorescence in situ hybridization (BAC-FISH) and cycling-primed in situ labeling (C-PRINS) techniques were evaluated for integration of physical and genetic maps of sunflower (Helianthus annuus L.). Single-site SSR markers were selected from three linkage groups of a high-density sunflower genetic map. This selection was based on previously identified QTL associated to S. sclerotiorum. These markers were used to select BACs contaning single copy sequences for BAC-FISH aplication. Blocking of highly dispersed repetitive sunflower sequences reduced unspecific hybridization, and allowed the detection of specific signals for BACs containing SSR markers HA4222 and HA2600, anchored to LG 16 and LG 10, respectively. Single-site... Tipo: Info:eu-repo/semantics/article Palavras-chave: Sunflower; BAC-FISH; C-PRINS; Single-copy sequences; Physical mapping. Ano: 2011 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452011000100003 Registros recuperados: 2 Primeira ... 1 ... Última

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