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Callus culture development of two varieties of Tagetes erecta and carotenoid production Electron. J. Biotechnol.
Benítez-García,Israel; Vanegas-Espinoza,Pablo Emilio; Meléndez-Martínez,Antonio J; Heredia,Francisco J; Paredes-López,Octavio; Del Villar-Martínez,Alma Angélica.
Background The properties of natural pigments, such as antioxidants, functional, medical, and nutraceutical, have demonstrated the advantages of these natural compounds over synthetic ones. Some products are accepted only when they are pigmented with natural, food-quality colorants: for example poultry products (manly marigold flower extracts). Carotenoids such as β-carotene, β-criptoxanthin and lutein are very attractive as natural food colorants due to their antioxidant and pro-vitamin activities which provide additional value to the target products. Marigold (Tagetes erecta) is an Asteraceous ornamental plant native to Mexico, and it is also important as a carotenoid source for industrial and medicinal purposes but nowadays its...
Tipo: Journal article Palavras-chave: 2 4-D; BA Cell development Lutein Marigold.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000300001
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Expression, purification and thermal stability evaluation of an engineered amaranth protein expressed in Escherichia coli Electron. J. Biotechnol.
Morales-Camacho,Jocksan I; Paredes-López,Octavio; Espinosa-Hernández,Edgar; Fernández Velasco,Daniel Alejandro; Luna-Suárez,Silvia.
Background: The acidic subunit of amarantin (AAC)-the predominant amaranth seed storage protein-has functional potential and its third variable region (VR) has been modified with antihypertensive peptides to improve this potential. Here, we modified the C-terminal in the fourth VR of AAC by inserting four VY antihypertensive peptides. This modified protein (AACM.4) was expressed in Escherichia coli. In addition, we also recombinantly expressed other derivatives of the amarantin protein. These include: unmodified amarantin acidic subunit (AAC); amarantin acidic subunit modified at the third VR with four VY peptides (AACM.3); and amarantin acidic subunit doubly modified, in the third VR with four VY peptides and in the fourth VR with the RIPP peptide...
Tipo: Journal article Palavras-chave: Globulin 11S; Protein expression; Protein engineering; Thermal stability.
Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000400007
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Fruit texture related changes and enhanced shelf-life through tomato root inoculation with Bacillus subtilis BEB-13BS Agrociencia
Mena-Violante,Hortencia G.; Cruz-Hernández,Adrés; Paredes-López,Octavio; Gómez-Lim,Miguel Á.; Olalde-Portugal,Víctor.
Bacillus subtilis BEB-13bs, a plant growth promoting rhizobacteria, has improved tomato firmness. Since firmer fruits are be expected to be more resistant to spoilage microorganisms and to have a longer shelf-life, root inoculation with this strain could be an alternative to extend tomato shelf-life. Thus, fruit texture-related changes were studied in tomato plants (Lycopersicon esculentum Mill.) inoculated with Bacillus subtilis BEB-13bs. Treatments were: 1) non-inoculated control treatment (CTL); 2) PGPR inoculated treatment (BS13). Evaluation was made at different ripening stages. Pericarp firmness and activity of poligalacturonase (PG), the major cell wall polyuronide degrading enzyme in tomato, were measured. The expression pattern of ripening-related...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lycopersicon esculentum; Aco gene expression; Firmness; PG activity; Quality; Ripening.
Ano: 2009 URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-31952009000600001
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