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Functional diversity of bacterial genes associated with aromatic hydrocarbon degradation in anthropogenic dark earth of Amazonia PAB
Germano,Mariana Gomes; Cannavan,Fabiana de Souza; Mendes,Lucas William; Lima,Amanda Barbosa; Teixeira,Wenceslau Geraldes; Pellizari,Vivian Helena; Tsai,Siu Mui.
The objective of this work was to evaluate the catabolic gene diversity for the bacterial degradation of aromatic hydrocarbons in anthropogenic dark earth of Amazonia (ADE) and their biochar (BC). Functional diversity analyses in ADE soils can provide information on how adaptive microorganisms may influence the fertility of soils and what is their involvement in biogeochemical cycles. For this, clone libraries containing the gene encoding for the alpha subunit of aromatic ring-hydroxylating dioxygenases (α-ARHD bacterial gene) were constructed, totaling 800 clones. These libraries were prepared from samples of an ADE soil under two different land uses, located at the Caldeirão Experimental Station - secondary forest (SF) and agriculture (AG) -, and the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Catabolic genes; Genetic diversity; Land use systems; Microbial ecology; Tropical soils.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2012000500004
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Detection of Cryptosporidium spp. Oocysts in raw sewage and creek water in the city of São Paulo, Brazil BJM
Farias,Eveline Wilma Coutinho; Gamba,Rosa C.; Pellizari,Vivian Helena.
The protozoan parasite Cryptosporidium has emerged as one of the most important contaminants of water, causing waterborne outbreaks of gastroenteritis worldwide. To monitor and understand the public health significance of this pathogen in environmental samples, several methods have been developed to isolate and detect Cryptosporidium oocysts. The purpose of this study was to perform the first investigation on the presence of Cryptosporidium spp. oocysts in raw sewage and creek water in the city of São Paulo, Brazil. The oocysts were concentrated by flocculation and membrane filtration. The results showed the occurrence of Cryptosporidium spp. in all wastewater samples analyzed, indicating a possible risk for dissemination of these pathogens in aquatic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cryptosporidium spp.; Sewage; Creek waters.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000100008
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