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Coelho,Stefania Vilas Boas; Rosa,Sttela Dellyzete Veiga Franco da; Clemente,Aline da Consolação Sampaio; Pereira,Cristiane Carvalho; Figueiredo,Madeleine Alves de; Reis,Leandro Vilela. |
ABSTRACT Preservation of the quality of coffee seeds is hindered by their intermediate behavior in storage. However, long-term storage at sub zero temperatures may be achieved by adjusting the water content of the seeds. The aim of this study was to evaluate the tolerance of coffee seeds to freezing, in relation to physiological and enzymatic modifications. Coffee seeds were dried in two manners, rapid and slow, to water contents of interest, 0.67, 0.43, 0.25, 0.18, 0.11, and 0.05 g H2O g-¹ dw (dry basis). After drying, the seeds were stored at a temperature of -20 ºC and of 86 ºC for 24 hours and for 12 months, and then compared to seeds in cold storage at 10 ºC. The seeds were evaluated through calculation of percentage of normal seedlings, percentage of... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Drying; Silica gel; Saturated saline solutions; Freezing; Antioxidant enzymes.. |
Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542017000300312 |
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Figueiredo,Madeleine Alves de; Rosa,Sttela Dellyzete Veiga Franco da; Ricaldoni,Marcela Andreotti; Pereira,Cristiane Carvalho; Coelho,Stefânia Vilas Boas; Silva,Luciano Coutinho. |
ABSTRACT Cryopreservation is a technique that may potentially conserve the germplasm of species of the Coffea genus for an indeterminate time. The aim of this study was to evaluate the physiological, biochemical and ultrastructural characteristics of cryopreserved seeds of Coffea arabica L., cultivar Catucaí amarelo IAC 62, which was subjected to different protocols regarding dehydration, precooling, cooling, rewarming and cathode water use. According to each protocol, the seeds were subjected to fast or slow drying to moisture contents of 17 or 20% (wet basis), cooled in different ways, and then immersed in liquid nitrogen for 24 hours. Different rewarming times in a water bath were also used. Physiological, biochemical and ultrastructural analyses were... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cathodic water; Dehydration; Long-term conservation; Ultrafast cooling.. |
Ano: 2021 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542021000100203 |
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