Home Itens selecionados Provedores de dados OAI Créditos Sobre Ajuda

			Busca avançada
	Provedor de dados Electron. J. Biotechnol. (3) Autor Petruccelli,Silvana (3) Alvarez,María Alejandra (1) Cazzola,María Laura (1) Giulietti,Ana María (1) Martínez,Carolina (1) Molina,María Isabel (1) Otegui,Marisa (1) Mais... Palavra-chave Bt maize (1) Catalytic antibodies (1) Developing sunflower seeds (1) Genetically modified organisms (1) In vitro cultures (1) Molecular farming (1) RR-soybean (1) Recombinant antibodies (1) Semi-quantitative PCR (1) Storage proteins (1) Tobacco (1) Transgenic food (1) Transport pathway (1) Vacuolar sorting (1) Vacuolar sorting receptor (1) Mais... Tipo do documento Journal article (3) Ano 2006 (2) 2005 (1) País Chile (3) Idioma Inglês (3)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 3 Primeira ... 1 ... Última Expression of the antibody 14D9 in Nicotiana tabacum hairy roots Electron. J. Biotechnol. Martínez,Carolina; Petruccelli,Silvana; Giulietti,Ana María; Alvarez,María Alejandra. Nicotiana tabacum hairy roots that express the antibody 14D9 were established. The 14D9 antibody yield obtained after 20 days of culture was 5.95 μg 14D9ml-1. The addition of the reticulum endoplasmic retention sequence KDEL demonstrated a positive effect over the intracellular 14D9 amounts with a yield increase up to 20.82 µg ml-1. DMSO increased the antibody amount in the biomass from 20.00 to 64.03 µg ml-1 while PVP (at 1.5 gl-1) and gelatine (at 5.0 gl-1) increased total 14D9 amounts in the culture medium to 25 µg and 14 µg respectively Tipo: Journal article Palavras-chave: Catalytic antibodies; In vitro cultures; Molecular farming; Recombinant antibodies; Tobacco. Ano: 2005 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000200006 Semiquantitative analysis of genetically modified maize and soybean in food Electron. J. Biotechnol. Cazzola,María Laura; Petruccelli,Silvana. The aim of this study was to analyze quantitatively the presence of genetically modified organism in food with different composition and degree of processing. Total DNA was extracted by Dellaporta's method and GMO analysis was performed using two consecutives PCR reactions with specie specific primers (IVR and LE), screening primers (35S) and transgen specific primers (CRY and EPSPS). The quantification within the sensitivity establish by the EU was possible only in some foods (ice-cream, flours, soybean isolates and concentrates, starch). Samples with high lipid content or subjected to intense thermal treatments (such as some snacks, mayonnaise, creamy soup) could not be amplified mainly due to the presence of PCR inhibitors. Therefore the method was... Tipo: Journal article Palavras-chave: Bt maize; Genetically modified organisms; Semi-quantitative PCR; RR-soybean; Transgenic food. Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000300028 Sunflower storage proteins are transported in dense vesicles that contain proteins homologous to the pumpkin vacuolar sorting receptor PV 72 Electron. J. Biotechnol. Molina,María Isabel; Otegui,Marisa; Petruccelli,Silvana. Storage proteins are transported to a special storage compartments in seeds by Golgi dependent or independent pathways depending on the plant species. The aim of this work was to study the sunflower storage protein transport pathway and identified component of the sorting machinery. Immature sunflower seeds were analyzed by subcellular fractionation (using percoll and sucrose gradients) and electron microscopy. The vesicles isolated with percoll, have precursors of 11S globulins, α-TIP, δ-TIP, BiP, and two proteins that have homology to the pumpkin vacuolar sorting receptor PV72. Sucrose isolated vesicles have the same composition than percoll ones, except for the lack of BiP and the presence of only one protein that has reactivity with... Tipo: Journal article Palavras-chave: Developing sunflower seeds; Storage proteins; Transport pathway; Vacuolar sorting; Vacuolar sorting receptor. Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000300029 Registros recuperados: 3 Primeira ... 1 ... Última

Empresa Brasileira de Pesquisa Agropecuária - Embrapa Todos os direitos reservados, conforme Lei n° 9.610 Política de Privacidade Área restrita		Embrapa Parque Estação Biológica - PqEB s/n° Brasília, DF - Brasil - CEP 70770-901 Fone: (61) 3448-4433 - Fax: (61) 3448-4890 / 3448-4891 SAC: https://www.embrapa.br/fale-conosco