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Isoenzyme and molecular approach for authenticating and monitoring of animal cell lines Anais da ABC (AABC)
ARAÚJO,SHIRLEY B. DE; PATRICIO,GIANCARLO F.; SIMONI,ISABELA C.; RIVAS,ELIANA B.; FERNANDES,MARIA JUDITE B..
Abstract: Authentication of cell lines is of paramount importance to validate the results from their use in biomedical research. Although isoenzyme polymorphism is the standard method, molecular methods based on mitochondrial DNA (mtDNA) have been developed to replace it. The aim of this study was the improvement of our isoenzyme electrophoretic analysis and the validation of one molecular technique targeted at mtDNA for the authentication of our animal cell lines. The combined method of cellular lysing through osmotic shock, followed by freezing-thawing in N2 to obtain isoenzyme extracts, and with 42 × 106 cells maintained the best efficiency. The superior electrophoretic conditions were PAGE run at 200 V. All cell lines had isoenzymatic mobility...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Authentication; Cell line; DNA barcode; Isoenzyme.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000500625
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