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Assessment of molecular recognition element for the quantification of human epidermal growth factor using surface plasmon resonance 69
Rosti,Ira Amira; Ramanan,Ramakrishnan Nagasundara; Ling,Tau Chuan; Ariff,Arbakariya B.
Background: A method for the selection of suitable molecular recognition element (MRE) for the quantification of human epidermal growth factor (hEGF) using surface plasmon resonance (SPR) is presented. Two types of hEGF antibody, monoclonal and polyclonal, were immobilized on the surface of chip and validated for its characteristics and performance in the quantification of hEGF. Validation of this analytical procedure was to demonstrate the stability and suitability of antibody for the quantification of target protein. Results: Specificity, accuracy and precision for all samples were within acceptable limit for both antibodies. The affinity and kinetic constant of antibodies-hEGF binding were evaluated using a 1:1 Langmuir interaction model. The model...
Tipo: Journal article Palavras-chave: Antibody; BIAcore; Biosensors; Epidermal growth factor; Validation.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000600015
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