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	Provedor de dados Electron. J. Biotechnol. (2) Autor Lakshmanan,Venkatachalam (2) Neelwarne,Bhagyalakshmi (2) Reddampalli Venkataramareddy,Sreedhar (2) Gokare Aswathanarayana,Ravishankar (1) Rudrappa,Thimmaraju (1) Palavra-chave Aspergillus (1) Calcium (1) Culture filtrate (1) Dried cell powder (1) Elicitor (1) Glutathione (1) Growth regulators (1) Methyljasmonate (1) Musa (1) Protocol (1) Rhizophus (1) Shoot cultures (1) Somaclonal variation (1) Thidiazuron (1) Mais... Tipo do documento Journal article (2) Ano 2007 (1) 2006 (1) País Chile (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Molecular analysis of genetic stability in long-term micropropagated shoots of banana using RAPD and ISSR markers Electron. J. Biotechnol. Lakshmanan,Venkatachalam; Reddampalli Venkataramareddy,Sreedhar; Neelwarne,Bhagyalakshmi. A large number of micropropagated plantlets of banana, Musa acuminata var. Nanjanagudu Rasabale (NR), that were developed from axillary shoot bud explants over 10 years ago were screened for genetic variation, if any, using RAPD (Random Amplified Polymorphic DNA) and ISSR (Inter-Simple Sequence Repeats) markers. Of the 4000 in vitro plantlets, 11 were used for screening that involved shoot cultures with distinct variation in morphological characteristics (morphotypes). Similarly, the mother maintained in the field was also subjected for genetic analysis. Out of the 50 RAPD and 25 ISSR primers screened, 30 RAPD and 5 ISSR primers produced totally 424 clear, distinct and reproducible band classes resulting in a total of 5088 bands where the banding patterns... Tipo: Journal article Palavras-chave: Growth regulators; Musa; Protocol; Shoot cultures; Somaclonal variation. Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000100010 Elicitation of peroxidase activity in genetically transformed root cultures of Beta vulgaris L. Electron. J. Biotechnol. Rudrappa,Thimmaraju; Neelwarne,Bhagyalakshmi; Lakshmanan,Venkatachalam; Reddampalli Venkataramareddy,Sreedhar; Gokare Aswathanarayana,Ravishankar. Genetically transformed roots of red beet produce copious levels of peroxidase (POD) - a multifunctional enzyme with a number of commercial applications. In an effort to elicit the POD activity, the cultures were treated with biotic elicitors such as dry cell powders of microbial cultures (0.1-0.5% w/v) and the respective culture filtrates (1-5% v/v). Similarly, abiotic elicitors, particularly metal ions (2-8 folds of that present in the nutrient medium), the plant hormone Thidiazuron (at 0.25-1 ppm) and other bio-molecules such as Glutathione (at 0.5-10 mM) and Methyl jasmonate (at 20-100 µM) were used. It was observed that dry cell powder of Candida versatilis significantly elicited the enzyme activity (3.52-fold higher than the control) followed by... Tipo: Journal article Palavras-chave: Aspergillus; Calcium; Culture filtrate; Dried cell powder; Elicitor; Glutathione; Methyljasmonate; Rhizophus; Thidiazuron. Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000500006 Registros recuperados: 2 Primeira ... 1 ... Última

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