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Association between molecular markers and behavioral phenotypes in the immatures of a butterfly Genet. Mol. Biol.
De Nardin,Janaína; Buffon,Vanessa; Revers,Luís Fernando; de Araújo,Aldo Mellender.
Abstract Newly hatched caterpillars of the butterfly Heliconius erato phyllis routinely cannibalize eggs. In a manifestation of kin recognition they cannibalize sibling eggs less frequently than unrelated eggs. Previous work has estimated the heritability of kin recognition in H. erato phyllis to lie between 14 and 48%. It has furthermore been shown that the inheritance of kin recognition is compatible with a quantitative model with a threshold. Here we present the results of a preliminary study, in which we tested for associations between behavioral kin recognition phenotypes and AFLP and SSR markers. We implemented two experimental approaches: (1) a cannibalism test using sibling eggs only, which allowed for only two behavioral outcomes (cannibal and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Kin discrimination; Caterpillar-egg cannibalism; Lepidoptera; Heliconius; AFLP.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000200243
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New biotechnological tools to accelerate scab-resistance trait transfer to apple Genet. Mol. Biol.
Cusin,Roberta; Revers,Luís Fernando; Maraschin,Felipe dos Santos.
Abstract Apple is a fruit crop cultivated worldwide. Apple orchards are exposed to a diverse set of environmental and biological factors that affect the productivity and sustainability of the culture. Many of the efforts and costs for apple production rely on reducing the incidence of fungal diseases, and one of the main diseases is apple scab caused by the fungus Venturia inaequalis. The economic impact of scab on apple productivity has guided many breeding programs to search for cultivars resistant to apple scab. Introgression from wild relatives has been successful to some extent, and genetic engineering for resistant cultivars has even been employed. This review presents the techniques used to the present time to obtain pathogen-resistant apple...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Malus; Venturia inaequalis; Biotic stress; Transformation.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000200305
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Uso prático de marcadores moleculares para seleção assistida no melhoramento de uvas de mesa apirênicas Rev. Bras. Frutic.
Revers,Luís Fernando; Lampe,Vanessa Sawatzky; Oliveira,Paulo Ricardo Dias de; Camargo,Umberto Almeida; Lima,Júlio César de.
A hipótese mais bem aceita atualmente para explicar a genética complexa da estenoespermocarpia, observada na videira, indica que a expressão deste fenótipo é controlada por três genes recessivos, independentemente herdados e controlados por um gene regulador dominante (sdI). Em estudo anterior, Lahogue et al. (1998) identificaram um marcador RAPD (Random Amplified Polymorphic DNA) ligado ao gene sdI e utilizaram-no para desenvolver um SCAR (Sequence Characterized Amplified Region) co-dominante denominado SCC8, que pode distinguir, em uma progênie, indivíduos com semente como também selecionar indivíduos apirênicos. Neste trabalho, são apresentados resultados da avaliação do potencial de aplicação do marcador molecular SCAR SCC8 para seleção assistida do...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Vitis; Marcador molecular; Seleção assistida; Apirenia.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452006000100029
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Developing a rapid, efficient and low cost method for rapid DNA extraction from arthropods Ciência Rural
Mega,Nicolás Oliveira; Revers,Luís Fernando.
Here, it is presented a rapid and efficient method to obtain good quality DNA from small samples of arthropod tissues generating low quantities of hazardous wastes. This new method was compared with another homemade protocol using phenol and other two commercial kits. The quality of DNA obtained was checked by spectrophotometer and evaluated by an AFLP assay. Low shearing DNA was obtained from all samples and the best readings were observed to DNA recollected with the new method. The AFLP assay indicated that DNA obtained with all methods were suitable for use in molecular biology techniques sensitive to contaminants. However, homemade protocols were more efficient in recollect DNA than commercial kits, without lose any quality of samples. Also, they were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Arthropod DNA; High quality DNA; Molecular biology; AFLP.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782011000900012
Registros recuperados: 4
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