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| Sera,Tumoru; Ruas,Paulo Maurício; Ruas,Claudete de Fátima; Diniz,Leandro Eugênio Cardamone; Carvalho,Valdemar de Paula; Rampim,Leandro; Ruas,Eduardo Augusto; Silveira,Sheila Recepute da. |
| Knowledge of the genetic variability among genotypes is important for the transfer of useful genes and to maximize the use of available germplasm resources. This study was carried out to assess the genetic variability of 14 elite Coffea arabica cultivars using random amplified polymorphic DNA (RAPD) associated with a prior digestion of genomic DNA with restriction endonucleases. The accessions were obtained from the Coffea collection maintained at the Instituto Agronômico do Paraná (IAPAR), located in Londrina, Paraná, Brazil. Twenty-four informative RAPD primers, used in association with restriction enzymes, yielded 330 reproducible and scorable DNA bands, of which 224 (68%) were polymorphic. The amplified products were used to estimate the genetic... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Coffea arabica; RAPD; Restriction endonucleases. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000100010 |
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| Medri,Cristiano; Medri,Moacyr Eurípedes; Ruas,Eduardo Augusto; Souza,Luiz Antônio de; Medri,Paulo Souza; Sayhun,Sandra; Bianchini,Edmilson; Pimenta,José Antônio. |
| Visando contribuir para o conhecimento das respostas morfoanatômicas de espécies arbóreas à hipoxia, efeitos do alagamento em órgãos vegetativos de Aegiphila sellowiana foram estudados. Indivíduos jovens foram mantidos em substrato drenado e alagado por 50 e 80 dias. Houve mortalidade de 40% das plantas alagadas. As sobreviventes produziram fissuras peridérmicas na base do hipocótilo, raízes diageotrópicas e lenticelas hipertrofiadas em hipocótilos e raízes. Em plantas alagadas: (1) as raízes secundárias apresentaram maior diâmetro, córtex mais espesso, células corticais maiores, espaços intercelulares maiores, cilindro vascular e elementos de vaso menores; (2) as raízes primárias em estrutura secundária e hipocótilos apresentaram súber e feloderme mais... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Anatomia ecológica; Mata ciliar; Hipoxia; Tolerância à inundação. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062011000200020 |
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| Diniz,Leandro Eugênio Cardamoni; Ruas,Claudete de Fátima; Carvalho,Valdemar de Paula; Torres,Fabrício Medeiros; Ruas,Eduardo Augusto; Santos,Melissa de Oliveira; Sera,Tumoru; Ruas,Paulo Maurício. |
| The genetic variability of 40 accessions of_C. arabica was evaluated using a combination of the random amplified polymorphic DNA (RAPD) technique and restriction digestion of genomic DNA. The genetic variability and the relatedness among all accessions were initially evaluated using 195 RAPD primers which revealed a very low level of genetic variation. To improve the efficiency in the detection of polymorphism, the genomic DNA of all accessions were submitted to digestion with restriction endonucleases prior to PCR amplification. A total of 24 primers combined with restriction digestion of DNA rendered 318 bands, of which 266 (83.65%) were polymorphic. The associations among genotypes were estimated using UPGMA-clustering analysis. The accessions were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Coffea arabica; Genetic variability; RAPD; Restriction endonucleases. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000500002 |
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| Sahyun,Sandra Aparecida; Ruas,Eduardo Augusto; Ruas,Claudete de Fátima; Medri,Cristiano; Souza,José Roberto Pinto de; Johansson,Loana Aparecida Pereira da Silva; Miranda,Luíz Vicente; Ruas,Paulo Maurício. |
| Three populations of Maytenus aquifolium from Monte Alegre farm, Telemaco Borba county, Paraná, Brazil were analyzed by RAPD markers. A total of 13 primers were applied wich yielded 283 well amplified markers in all the studied populations (Mortandade, Vila Preta and Trinita), producing different values of gene diversity and polymorphic loci. The analysis of molecular variance (AMOVA) indicated that 21.77% of the genetic variation was among the population. Pairwise F ST analysis showed that the most divergent populations were closer geographically, demonstrating that other factors such as different soil types could explain this variation. Bayesian analysis for K number of clusters and the Principal Coordinate indicated that these three populations were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Conservation; Genetic diversity; Maytenus aquifolium; RAPD; Populations. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000500005 |
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