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Co-localization of GSTP1 and JNK in transitional cell carcinoma of urinary bladder Genet. Mol. Biol.
Pljesa-Ercegovac,Marija; Savic-Radojevic,Ana; Kravic-Stevovic,Tamara; Bumbasirevic,Vladimir; Mimic-Oka,Jasmina; Simic,Tatiana.
Transitional cell carcinoma (TCC) of urinary bladder belongs to glutathione S-transferase P1 (GSTP1) overexpressing tumors. Upregulated GSTP1 in TCC is related to apoptosis inhibition. This antiapoptotic effects of GSTP1 might be mediated through protein:protein interaction with c-Jun NH2-terminal kinase (JNK). Herein, we analyzed whether a direct link between GSTP1 and JNK exists in TCC. The presence of GSTP1/JNK complexes was analyzed by immunoprecipitation and Western blotting in 20 TCC specimens, obtained after surgery. Co-localization of GSTP1 and JNK was also investigated in the 5637 TCC cell line by immunofluorescence confocal microscopy. By means of immunoprecipitation we show for the first time the presence of GSTP1/JNK complexes in all TCC...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Glutathione S-transferase; JNK; TCC; Co-localization.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300013
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