|
|
|
|
|
| Scodro,Regiane Bertin de Lima; Reinhold-Castro,Kárin Rosi; Dias-Sversutti,Alessandra de Cassia; Neitzke-Abreu,Herintha Coeto; Membrive,Norberto de Assis; Kühl,João Balduíno; Silveira,Thaís Gomes Verzignassi; Teodoro,Ueslei. |
| The purpose of this work was to verify the occurrence of Leishmania in naturally infected sandflies. The insects were collected with Falcão, Shannon and HP light-traps, in Doutor Camargo and Maringá municipalities between November 2004 and October 2005. Of the 11,033 sandflies collected in Doutor Camargo, 2,133 surviving females were dissected, particularly those of the Nyssomyia neivai species (86.87%). In Maringá, 136 sandflies were collected, of which 79 N. whitmani females and 1 Migonemyia migonei female were dissected. The dissected insects were identified and stored in the pools of 10 specimens. The PCR was carried out on 1,190 females of N. neivai and 190 of N. whitmani from Doutor Camargo, and on 30 of N. whitmani from Maringá, using the primers... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cutaneous leishmaniasis; Sandflies; Dissection; Polymerase chain reaction. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000300007 |
| |
|
|
| Leite,Clariana Akemi Kariya; Oizumi,Karina Yoshimi; Caleffi-Ferracioli,Katiany Rizzieri; Scodro,Regiane Bertin de Lima; Pádua,Rubia Andreia Falleiros de; Cardoso,Rosilene Fressatti; Pires,Claudia Terencio Agostinho; Siqueira,Vera Lucia Dias. |
| ABSTRACT The present study evaluated the antimicrobial susceptibility profile, β-lactamase production, and genetic diversity of Enterobacteriaceae, Pseudomonas aeruginosa, and Acinetobacter spp. using phenotypic identification, antimicrobial susceptibility testing, and β-lactamase phenotypic detection. Isolates were obtained from patients in an intensive care unit in a hospital in southern Brazil. Bacterial genomic DNA was extracted, followed by the genotypic detection of carbapenemases and enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR). Fifty-six isolates (26 Klebsiella pneumoniae, five Escherichia coli, three Enterobacter aerogenes, nine P. aeruginosa, and 13 Acinetobacter spp.) were evaluated. The phenotypic... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Enterobacteriaceae; Pseudomonas aeruginosa; Acinetobacter spp; Antimicrobial resistance; Beta-lactamases; Bacterial typing; Intensive Care Unit/study/Brazil; Drug-resistant bacteria.. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000200609 |
| |
|
|
| Meneguello,Jean Eduardo; Silva,João Vitor de Oliveira; Lima,Letícia Silva; Siqueira,Vera Lucia Dias; Scodro,Regiane Bertin de Lima; Caleffi-Ferracioli,Katiany Rizzieri; Cardoso,Rosilene Fressatti. |
| ABSTRACT Microscopy and bacterial culture are the main tools in the diagnosis of tuberculosis. Since the slow growth of Mycobacterium tuberculosis impairs rapid diagnosis strategies, especially in countries where the latter are the only available resources, the ongoing development of new and inexpensive tools based on mycobacterial metabolism optimizing growth detection with preliminary identification is greatly welcome. When compared to the other species from the M. tuberculosis complex, M. tuberculosis is a strong nitrate reducer. Current assay compares the nitrate reductase activity of M. tuberculosis from pulmonary specimens cultivated in nitrate-supplemented media. Fifty-five sputum samples were decontaminated and inoculated in conventional... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Tuberculosis; Mycobacteria/diagnosis/identification; Nitrate reductase. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000100602 |
| |
|
|
| Maltempe,Flaviane Granero; Baldin,Vanessa Pietrowski; Lopes,Mariana Aparecida; Siqueira,Vera Lúcia Dias; Scodro,Regiane Bertin de Lima; Cardoso,Rosilene Fressatti; Caleffi-Ferracioli,Katiany Rizzieri. |
| ABSTRACT Leprosy is a neglected tropical disease and an important public health problem, especially in developing countries. It is a chronic infectious disease that is caused by Mycobacterium leprae, which has a predilection for the skin and peripheral nerves. Although it has low sensitivity, slit-skin smear (SSS) remains the conventional auxiliary laboratory technique for the clinical diagnosis of leprosy. Polymerase chain reaction (PCR) is a molecular biology technique that holds promise as a simple and sensitive diagnostic tool. In the present study, the performance of two PCR methods, using different targets, PCR-LP and PCR-P, were compared with SSS with regard to leprosy diagnosis in a reference laboratory. M. leprae DNA was extracted from 106 lymph... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leprosy/clinical diagnosis; Polymerase chain reaction/clinical diagnosis use; Mycobacterium leprae. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000100018 |
| |
|
|
| Amaral,Renata Claro Ribeiro do; Caleffi-Ferracioli,Katiany Rizzieri; Demitto,Fernanda de Oliveira; Almeida,Aryadne Larissa de; Siqueira,Vera Lucia Dias; Scodro,Regiane Bertin de Lima; Leite,Clarice Queico Fujimura; Pavan,Fernando Rogério; Cardoso,Rosilene Fressatti. |
| The membrane-based efflux pump systems are recognized to have an important role in pathogenicity and drug resistance in Mycobacterium tuberculosis by the extrusion of toxic substrates and drugs from the inner bacillus. This study aimed to investigate the in vitro interaction of Verapamil (VP), an efflux pump inhibitor, with the classical first-line anti-tuberculosis drug isoniazid (INH) in resistant and susceptible M. tuberculosis clinical isolates. Seven multidrug-resistant (MDR), three INH monoresistant and four susceptible M. tuberculosis clinical isolates were tested for the INH and VP combination by modified Resazurin Microtiter Assay Plate (REMA). Fractional Inhibitory Concentration (FIC) and Modulation Factor (MF) were determined. The INH plus VP... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Tuberculosis; Multidrug-resistance; Efflux pumps; Efflux pumps inhibitors; Isoniazid. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100588 |
| |
|
|
|
