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Hydrogen peroxide induces a specific DNA base change profile in the presence of the iron chelator 2,2’ dipyridyl in Escherichia coli BJMBR
Felício,D.L.; Almeida,C.E.B.; Silva,A.B.; Leitão,A.C..
Pretreatment of Escherichia coli cultures with the iron chelator 2,2’-dipyridyl (1 mM) protects against the lethal effects of low concentrations of hydrogen peroxide (<15 mM). However, at H2O2 concentrations equal to or greater than 15 mM, dipyridyl pretreatment increases lethality and mutagenesis, which is attributed to the formation of different types of DNA lesions. We show here that pretreatment with dipyridyl (1 mM) prior to challenge with high H2O2 concentrations (≥15 mM) induced mainly G:C→A:T transitions (more than 100X with 15 mM and more than 250X with 20 mM over the spontaneous mutagenesis rate) in E. coli. In contrast, high H2O2 concentrations in the absence of dipyridyl preferentially induced A:T→T:A transversions (more than 1800X and more...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hydrogen peroxide; Dipyridyl; Mutagenesis; Iron; Fenton reaction.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001100003
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Antibacterial activity, chemical composition, and cytotoxicity of leaf's essential oil from Brazilian pepper tree (Schinus terebinthifolius, Raddi) BJM
Silva,A.B.; Silva,T.; Franco,E.S.; Rabelo,S.A.; Lima,E.R.; Mota,R.A.; Câmara,C.A.G. da; Pontes-Filho,N.T.; Lima-Filho,J.V..
The antibacterial potential of leaf's essential oil (EO) from Brazilian pepper tree (Schinus terebinthifolius Raddi) against staphylococcal isolates from dogs with otitis externa was evaluated. The minimum inhibitory concentration of EO ranged from 78.1 to 1,250 fg/mL. The oil was analyzed by GC and GC/MS and cytotoxicity tests were carried out with laboratory animals.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Essential oil; Brazilian pepper tree; Schinus terebinthifolius; Otitis externa; Staphylococcus.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000100023
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