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| Registros recuperados: 10 | |
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| Sofia,Silvia H.; Galindo,Bruno A.; Paula,Francine M.; Sodré,Leda M.K.; Martinez,Cláudia B.R.. |
| In this study, random amplified polymorphic DNA (RAPD) markers were applied to analyze the genetic diversity of samples of the Neotropical catfish Hypostomus ancistroides, collected from four sites (S1, S2, S3 and S4) along an urban stream in Southern Brazil. The 11 primers used in RAPD analysis amplified 147 loci, 76 (51.7%) of which were polymorphic. The proportions of polymorphic loci observed in the four samples were: 29.93% (S1), 31.97% (S2), 23.81% (S3) and 38.77% (S4). The average heterozygosity within sampling localities ranged from 0.1230 to 0.1526 and unbiased genetic distances ranged from 0.0253 to 0.0445. AMOVA partitioned 90.85% of the total variation within samples and 9.15% among samples. Excepting for the sample pair S1-S2 (phiST = 0.02784;... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Loricariid fish; Genetic diversity; RAPD; Siluriformes. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200027 |
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| Sofia,Silvia H.; Santos,Aline M. dos; Silva,Carlos R. M. da. |
| Species composition, relative abundance, seasonal changes in the species abundance and scent association of male Euglossini collected in a semi-deciduous forest fragment in the north of the State of Paraná, southern Brazil, were recorded. Euglossine males were collected twice a month, for twelve months, from 8:00 am to 3:00 pm. The scents eucalyptol, eugenol, vanillin, methyl salicylate and benzyl acetate were used as baits. A total of 434 males distributed among 3 genera and 9 species were attracted to the chemical baits. Eufriesea violacea (Blanchard, 1840) (49.8%), Eulaema nigrita Lepeletier, 1841 (23.0%) and Euglossa pleosticta Dressler, 1982 (13.8%) were dominant in number of individuals. Among the non-dominant species, Euglossa fimbriata Rebêlo... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Euglossinae; Euglossini; Forest fragment; Orchid bees; Scent baits. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0073-47212004000200015 |
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| Pascual,Amália N.T.; Suzuki,Karen M.; Almeida,Fernanda S.; Sodré,Leda M.K.; Sofia,Silvia H.. |
| In the current literature, information is scarce on which part of the adult insect body is suitable for isolation of genomic DNA for genetic analysis based on DNA-markers. In this study, we evaluated RAPD profiles generated from total genomic DNA isolated from distinct body parts (head, legs, thorax + wings and abdomen) of 12 males of Euglossa pleosticta Dressler. From the total of bands analyzed, 9.0% did not show reproducibility. Percent variations of bands in each body segment were: 1.1% (head); 0.4% (legs); 0.8% (thorax/wings) and 6.7% (abdomen). The much higher variation (chi2one sample = 10.27; df = 1; P < 0.01) in the RAPD profiles obtained by using DNA isolated from abdomen of the euglossine males suggests that this body part of adult insects... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Apoidea; Euglossini; Orchid bee; Euglossine bee; Molecular marker. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2006000600014 |
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| Sofia,Silvia H.; Suzuki,Karen M.. |
| O objetivo deste trabalho foi estudar as comunidades de abelhas Euglossina em três fragmentos de floresta semidecídua de tamanhos diferentes (8,5 ha, 86 ha e 2280 ha), na região de Londrina, norte do Paraná. De novembro de 2001 a abril de 2002, machos atraídos às iscas-odores de eucaliptol, eugenol e vanilina foram amostrados em coletas mensais, com 2h de duração. Foram coletados 245 indivíduos pertencentes a quatro gêneros e sete espécies. No fragmento maior foram coletados 152 machos pertencentes a seis espécies, no médio, 45 machos de cinco espécies e, no menor, 48 machos de sete espécies. Os índices de diversidade (H') e eqüidade de espécies (J') variaram de 1,21 a 1,42 e 0,62 a 0,86, respectivamente. A ordem de abundância das espécies variou nos três... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Abelha neotropical; Abelha-das-orquídeas; Isca-odor; Floresta semidecídua. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2004000600006 |
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| Apolinário-Silva,Caroline; Ferreira,Dhiego G.; Cavenagh,Analiza F.; Aprígio,Nícollas G. O.; Galindo,Bruno A.; Carlsson,Jens; Sofia,Silvia H.. |
| ABSTRACT Data on 15 novel microsatellite loci from the Neotropical fish Bryconamericus aff. iheringii are presented here. Analyses of 32 individuals from four different streams revealed 192 different alleles, ranging from four to 32 alleles per locus (mean of 12.8 per locus). Observed and expected heterozygosities ranged from 0.094 to 0.813 and 0.205 to 0.952, respectively. These loci showed high polymorphic information content and will be a resource for genetic studies of B. aff. iheringii. Furthermore, several loci also amplified other small Neotropical Characidae (Piabarchus stramineus and Piabina argentea) and should be useful for these species. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Genetic diversity; Lambarizinho; Molecular markers; Neotropical fish; SSR. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252018000100218 |
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| Galindo,Bruno A.; Ferreira,Dhiego G.; Apolinário-Silva,Caroline; Terra,Mariana C.; Aprígio,Nícollas G. O.; Ota,Renata R.; Ohara,Willian M.; Almeida,Fernanda S.; Sofia,Silvia H.. |
| ABSTRACT Brycon nattereri is an endangered Neotropical fish reported along conserved stretches of the upper Paraná, Tocantins and São Francisco rivers. Populations of this species have been very rare in some Paraná River sub basins. This study analyzes the genetic diversity and population structure of B. nattereri in a restricted area of occurrence recently identified in upper Paraná River basin. Seven microsatellite loci and 497 bp of D-Loop mitochondrial region were examined in 92 individuals from four points along the area of occurrence. Both molecular markers indicated a single population distributed along a stretch of the river approximately 80 km long. Although some of the data suggest an ancient bottleneck, current levels of genetic diversity (H E... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Conservation; Endangered species; Molecular markers; Population genetics; Pirapitinga. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252019000100208 |
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| Souza-Shibatta,Lenice; Ferreira,Dhiego G.; Santos,Kátia F.; Galindo,Bruno A.; Shibatta,Oscar A.; Sofia,Silvia H.; Giacomin,Renata M.; Bastos,Douglas A.; Mendes-Júnior,Raimundo N. G.; Santana,Carlos David de. |
| Abstract Fourteen novel microsatellite loci are described and characterized in two species of electric eels, Electrophorus variiand E. voltaifrom floodplains and rivers of the Amazon rainforest. These loci are polymorphic, highly informative, and have the capacity to detect reliable levels of genetic diversity. Likewise, the high combined probability of paternity exclusion value and low combined probability of genetic identity value obtained demonstrate that the new set of loci displays suitability for paternity studies on electric eels. In addition, the cross-amplification of electric eel species implies that it may also be useful in the study of the closely related E. electricus, and to other Neotropical electric fishes (Gymnotiformes) species as tested... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Amazon Rainforest; Electrophorus; Genetic diversity; Gymnotiformes; SSR. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252020000400202 |
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| Registros recuperados: 10 | |
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