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| Souza-Shibatta,Lenice; Ferreira,Dhiego Gomes; Oliveira,Claudio; Almeida,Fernanda Simões de; Shibatta,Oscar Akio; Sofia,Silvia Helena. |
| Thirteen microsatellite loci were isolated and characterized in Microglanis cottoides. Of these, two were monomorphic and 11 were polymorphic. These polymorphic loci tested on 24 individuals from a wild population produced a total of 108 different alleles, with levels of variability high, ranging from 2 to 20, with an average of 8.3 alleles per locus. The observed and expected heterozygosity ranged from 0.125 to 0.958 and from 0.119 to 0.931, respectively. A high combined probability of paternity exclusion value and a low probability combined genetic identity value obtained show that the set of loci described herein displays good suitability for paternity studies and differentiation of M. cottoides. Additionally, all thirteen microsatellite primers... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Brazilian Eastern basin; Catfish; Enriched genomic library; Genetic marker; SSR. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252013000300581 |
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| Souza-Shibatta,Lenice; Pezenti,Larissa Forim; Ferreira,Dhiego Gomes; Almeida,Fernanda Simões de; Sofia,Silvia Helena; Shibatta,Oscar Akio. |
| Specimens of Pimelodellacaptured in the Miranda River, Pantanal of Mato Grosso do Sul State, present morphological features that could indicate at least four species. Therefore, karyotype analysis and molecular biology provided evidence that they were only two species, one showing 2n = 46, and the other, 2n = 52 chromosomes, with only 18% genetic similarity. The morphological analysis evidenced that the dorsal filament is a male characteristic and that the upper lobe of the caudal fin was variable and might or might not be elongated in both species. With respect to morphometric characters, the formation of two groups was evident, but with a small overlap of specimens between them. Among the species with filaments on the dorsal fin observed in the Pantanal,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cytotaxonomy; Cytogenetics; Multivariate morphometrics; RAPD. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252013000100101 |
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| Silveira,Guilherme do Carmo; Nascimento,Anderson Machado; Sofia,Silvia Helena; Augusto,Solange Cristina. |
| Diversity of the euglossine bee community (Hymenoptera, Apidae) of an Atlantic Forest remnant in southeastern Brazil. Euglossine bees, attracted to scent baits of cineole, eugenol and vanillin, were collected with entomological nets, from December 1998 to November 1999. Samplings were carried out once a month simultaneously by two collectors positioned in two different sites in an Atlantic Forest remnant in northeastern São Paulo state, Brazil. A total of 859 male euglossine bees, belonging to 13 species and four Euglossini genera were collected. Of the total sample, 506 (12 species) males were captured at site A and 353 (10 species) were collected at site B.In both sites, Euglossa pleosticta Dressler, 1982 was the most abundant species (45.79%), followed... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Neotropical insects; Orchid bees; Semidecidual seasonal forest. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262011000100017 |
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| Ono,Elisabete Yurie Sataque; Fungaro,Maria Helena Pelegrinelli; Sofia,Silvia Helena; Miguel,Tatiana de Ávila; Sugiura,Yoshitsugu; Hirooka,Elisa Yoko. |
| In this study a total of 16 Fusarium verticillioides strains isolated from corn feed samples were characterized by fumonisin (FB) production and random amplified polymorphic DNA (RAPD). All the strains produced FB1 and FB2 with levels ranging from 2.41 to 3996.36 µg/g, and from 1.18 to 1209.91 µg/g, respectively. From the 16 F. verticillioides strains, four were identified as low (3.59 to 1289.84 µg/g), eight as intermediate (>1289.84 to 3772.44 µg/g) and four strains as high (>3772.44 µg/g) fumonisin producers. From the total of 105 loci amplified, 60 (57.14%) were polymorphic. RAPD analysis showed very similar patterns among low, moderate and high fumonisin-producing strains. Although RAPD markers were capable of discriminating the different F.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fumonisin; Fusarium verticillioides; Genetic variability; RAPD. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000400026 |
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| Toledo,Vagner de Alencar Arnaut de; Fritzen,Adriano Ernesto de Toledo; Neves,Carolina Antunes; Ruvolo-Takasusuki,Maria Claudia Colla; Sofia,Silvia Helena; Terada,Yoko. |
| The present study was carried out to survey the bees as visitors to melliferous flora in the region of Maringá, state of Paraná, Brazil. A total of 331 insects were captured, and the fauna comprised 39.88% Trigona spinipes, 38.37% Apis mellifera, 8.16% Tetragonisca angustula, 3.93% Halictidae, 1.21% Megachilidae, 2.42% Anthophoridae, and 3.32% other Hymenoptera. Eleven plant species from nine families were observed. The four families most frequently visited by A. mellifera were Pontederiaceae (93.53%), Sterculiaceae and Polygoniaceae (47.22%), Apocynaceae and Apiaceae (42.86%). The families most visited by T. spinipes were Lamiaceae (64.70%), Apocynaceae (57.14%), Sterculiaceae (51.85%) and Anacardiaceae (48.39%), and the families most visited by T.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Stingless bees; Pollination; Melliferous flora. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000400025 |
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