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Does the chromosomal position of 35S rDNA sites influence their transcription? A survey on Nothoscordum species (Amaryllidaceae) Genet. Mol. Biol.
Báez,Mariana; Souza,Gustavo; Guerra,Marcelo.
Abstract 35S ribosomal DNA (rDNA) sites are the regions where the ribosomal genes 18S, 5.8S and 25S, responsible for the formation of the nucleoli, are found. The fact that rDNA sites have non-random distribution on chromosomes suggests that their positions may influence their transcription. To identify if the preferentially transcribed rDNA sites occupy specific position, six species (nine cytotypes) of the genus Nothoscordum were analyzed using two different techniques to impregnate the nucleolar organizer regions (NORs) with silver nitrate. Both techniques strongly stained NORs, but one of them also stained the proximal region of all chromosomes, suggesting the existence of another group of argentophilic proteins in this region. In species with rDNA...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Nucleolus; Ribosomal genes; Preferential activation of NORs; Nothoscordum.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100305
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Low cytomolecular diversification in the genus Stylosanthes Sw. (Papilionoideae, Leguminosae) Genet. Mol. Biol.
Franco,Ana Luiza; Figueredo,Amanda; Pereira,Lívia de Moraes; Sousa,Saulo Marçal de; Souza,Gustavo; Carvalho,Marcelo Ayres; Simon,Marcelo F.; Viccini,Lyderson Facio.
Abstract Stylosanthes (Papilionoideae, Leguminosae) is a predominantly Neotropical genus with ~48 species that include worldwide important forage species. This study presents the chromosome number and morphology of eight species of the genus Stylosanthes (S. acuminata, S. gracilis, S. grandifolia, S. guianensis, S. hippocampoides, S. pilosa, S. macrocephala, and S. ruellioides). In addition, staining with CMA and DAPI, in situ hybridization with 5S and 35S rDNA probes, and estimation of DNA content were performed. The interpretation of Stylosanthes chromosome diversification was anchored by a comparison with the sister genus Arachis and a dated molecular phylogeny based on nuclear and plastid loci. Stylosanthes species showed 2n = 20, with low...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Arachis; Cytogenetics; Evolution; Leguminosae; Stylosanthes.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100306
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