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JING, Zhang; MAGONA, Joseph W; SAKURAI, Tatsuya; THEKISOE, Oriel M. M; OTIM, Charles P; SUGIMOTO, Chihiro; INOUE, Noboru; 井上, 昇. |
Prevalence of bovine trypanosomosis was determined from a total of 203 blood samples collected from Butaleja district, eastern Uganda. All samples were examined by microhematocrit centrifuge test (MHC), PCR and ELISA. ELISA was performed in accordance with the OIE standard procedures using Trypanosoma brucei gambiense procyclic form crude antigens. PCR were utilized to identify the species and the subspecies of trypanosome. The overall prevalence of bovine African trypanosomosis was 8.9% by MHC, and 45.3% by the ELISA. Since substantial number (12 out of 18) of MHC positive samples were negative in the PCR tests, we could not conclude the most epidemic trypanosome species in the studied area. Nevertheless, the PCR results suggests that the most prevalent... |
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Palavras-chave: Bovine; Prevalence; Trypanosome; Uganda; Zoonotic parasite. |
Ano: 2009 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2690 |
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THEKISOE, Oriel M. M; BAZIE, Raoul S. B; CORONEL-SERVIAN, Andrea M; SUGIMOTO, Chihiro; KAWAZU, Shin-ichiro; INOUE, Noboru; 井上, 昇. |
This study evaluated the stability of LAMP reagents when stored at 25C and 37C, and also assessed its detection efficiency on different DNA template preparations. Accordingly, LAMP using reagents stored at 25C and 37C amplified DNA of in vitro cultured T. b. brucei (GUTat 3.1) from day 1 to day 15 of reagent storage. There were no significant differences (P>0.05) in detection sensitivity of LAMP among the reagents stored at 25C, 37C and –20C (recommended storage temperature). LAMP using the reagents stored at above-mentioned temperatures amplified serially diluted DNAs (genomic DNA extracted by phenol-chloroform method, FTA card and hemolysed blood) of T. b. gambiense (IL2343) with high sensitivity. Reactions were conducted on the reagents stored... |
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Palavras-chave: Diagnosis; DNA template; LAMP; Parasitic disease; Trypanosoma. |
Ano: 2009 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2691 |
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