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| Souza,Alessandra A. de; Takita,Marco A.; Coletta-Filho,Helvécio D.; Targon,Maria Luisa P.N.; Carlos,Eduardo F.; Locali-Fabris,Eliane C.; Amaral,Alexandre M.; Freitas-Astúa,Juliana; Silva-Pinhati,Ana Carla O.; Boscariol-Camargo,Raquel L.; Berger,Irving J.; Rodrigues,Carolina M.; Reis,Marcelo S.; Machado,Marcos A.. |
| In order to understand the genetic responses resulting from physiological changes that occur in plants displaying citrus variegated chlorosis (CVC) symptoms, we adopted a strategy of comparing two EST libraries from sweet orange [Citrus sinensis (L.) Osbeck]. One of them was prepared with plants showing typical CVC symptoms caused by Xylella fastidiosa and the other with non-inoculated plants. We obtained 15,944 ESTs by sequencing the two cDNA libraries. Using an in silico hybridization strategy, 37 genes were found to have significant variation at the transcriptional level. Within this subset, 21 were up-regulated and 16 were down-regulated in plants with CVC. The main functional categories of the down-regulated transcripts in plants with CVC were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Sweet orange; EST; CVC; Compatible interaction. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500023 |
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| Quecini,Vera; Torres,Gisele A.M.; Rosa Jr,Vicente E. de; Gimenes,Marcos A.; Machado,Jeanne B. de M.; Figueira,Antonio V. de O.; Benedito,Vagner; Targon,Maria Luisa P.N.; Cristofani-Yaly,Mariângela. |
| Plant hormones play a crucial role in integrating endogenous and exogenous signals and in determining developmental responses to form the plant body throughout its life cycle. In citrus species, several economically important processes are controlled by phytohormones, including seed germination, secondary growth, fruit abscission and ripening. Integrative genomics is a powerful tool for linking newly researched organisms, such as tropical woody species, to functional studies already carried out on established model organisms. Based on gene orthology analyses and expression patterns, we searched the Citrus Genome Sequencing Consortium (CitEST) database for Expressed Sequence Tags (EST) consensus sequences sharing similarity to known components of hormone... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Defense responses; Development; Plant growth regulators. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500002 |
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| Freitas-Astúa,Juliana; Bastianel,Marinês; Locali-Fabris,Eliane C.; Novelli,Valdenice M.; Silva-Pinhati,Ana Carla; Basílio-Palmieri,Ana Carolina; Targon,Maria Luisa P.N.; Machado,Marcos A.. |
| Leprosis, caused by Citrus leprosis virus, cytoplasmic type (CiLV-C), is the main viral disease in the Brazilian citrus industry. This occurs because of the widespread source of inoculum and the year-round presence of the vector, the tenuipalpid mite Brevipalpus phoenicis, in citrus plants. In addition, while some Citrus species are resistant to CiLV-C, C. sinensis, the main cultivated species in the country, is extremely susceptible to the disease. The main objective of this work was to identify genes in C. sinensis cv. Pêra plants that were differentially expressed after the host was challenged with CiLV-C. In order to accomplish that, cDNA libraries were constructed from healthy and CiLV-inoculated sweet orange leaves. Two hundred and fifty-four genes... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: EST; Disease; CiLV; In silico hybridization; Sweet orange; Citrus sinensis. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500026 |
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| Rosa,Daniel D.; Campos,Magnólia A.; Targon,Maria Luisa P.N.; Souza,Alessandra A.. |
| Due to the economic importance of gummosis disease for the citriculture, studies on P. parasitica-Citrus interaction comprise a significant part in the Brazilian Citrus genome data bank (CitEST). Among them, two cDNA libraries constructed from two different growth conditions of the P. parasitica pathogen are included which has generated the PP/CitEST database (CitEST - Center APTA Citros Sylvio Moreira/IAC- Millennium Institute). Through this genomic approach and clustering analyses the following has been observed: out of a total of 13,285 available in the Phytophthora parasitica database, a group of 4,567 clusters was formed, comprising 2,649 singlets and 1,918 contigs. Out of a total of 4,567 possible genes, only 2,651 clusters were categorized; among... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Citrus disease; Elicitins; Plant pathogen; Gene expression profiles. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500028 |
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| Boscariol-Camargo,Raquel L.; Berger,Irving J.; Souza,Alessandra A.; Amaral,Alexandre M. do; Carlos,Eduardo F.; Freitas-Astúa,Juliana; Takita,Marco A.; Targon,Maria Luisa P.N.; Medina,Camilo L.; Reis,Marcelo S.; Machado,Marcos A.. |
| CitEST project resulted in the construction of cDNA libraries from different Citrus sp. tissues under various physiological conditions. Among them, plantlets of Rangpur lime were exposed to hydroponic conditions with and without water stress using PEG6000. RNA from roots was obtained and generated a total of 4,130 valid cDNA reads, with 2,020 from the non-stressed condition and 2,110 from the stressed set. Bioinformatic analyses measured the frequency of each read in the libraries and yielded an in silico transcriptional profile for each condition. A total of 40 contigs were differentially expressed and allowed to detect up-regulated homologue sequences to well known genes involved in stress response, such as aquaporins, dehydrin, sucrose synthase, and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Expressed sequence tags; Transcription; Rootstock; Drought; Molecular response. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500019 |
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| Cristofani-Yaly,Mariângela; Berger,Irving J.; Targon,Maria Luisa P.N.; Takita,Marco A.; Dorta,Sílvia de O.; Freitas-Astúa,Juliana; Souza,Alessandra A. de; Boscariol-Camargo,Raquel L.; Reis,Marcelo S.; Machado,Marcos A.. |
| Citrus is the most important fruit crop in Brazil and Citrus tristeza virus (CTV) is considered one of the most important pathogens of citrus. Most citrus species and varieties are susceptible to CTV infection. However, Poncirus trifoliata, a close relative of citrus, is resistant to the virus. In order to better understand the responses of citrus plants to the infection of CTV, we constructed expressed sequence tag (EST) libraries with tissues collected from Poncirus trifoliata plants, inoculated or not with Citrus tristeza virus at 90 days after inoculation, grafted on Rangpur lime rootstocks. We generated 17,867 sequence tags from Poncirus trifoliata inoculated (8,926 reads) and not (8,941 reads) with a severe CTV isolate. A total of 2,782 TCs... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Citrus; Disease resistance; Citrus tristeza virus; Biotic stress. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500025 |
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| Campos,Magnólia A.; Rosa,Daniel D.; Teixeira,Juliana Érika C.; Targon,Maria Luisa P.N.; Souza,Alessandra A.; Paiva,Luciano V.; Stach-Machado,Dagmar R.; Machado,Marcos A.. |
| In silico expression profiles, of the discovered 3,103 citrus ESTs putatively encoding for PR protein families (PR-1 to PR-17), were evaluated using the Brazil citrus genome EST CitEST/database. Hierarchical clustering was displayed to identify similarities in expression patterns among citrus PR-like gene families (PRlgf) in 33 selected cDNA libraries. In this way, PRlgf preferentially expressed by organ and citrus species, and library conditions were highlighted. Changes in expression profiles of clusters for each of the 17 PRlgf expressed in organs infected by pathogens or drought-stressed citrus species were displayed for relative suppression or induction gene expression in relation to the counterpart control. Overall, few PRlgf showed expression 2-fold... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Citrus; Functional genome; Ests; Gene expression profiles; Pathogenesis-related proteins; Defense genes. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500020 |
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| Eiras,Marcelo; Targon,Maria Luisa P.N.; Fajardo,Thor V.M.; Flores,Ricardo; Kitajima,Elliot W.. |
| Viroids, non-protein-coding small (246-401 nt) circular single-stranded RNAs with autonomous replication, are currently classified into two families. Within the family Pospiviroidae, Citrus exocortis viroid (CEVd) belongs to the genus Pospiviroid while Hop stunt viroid (HSVd) is the single member of the genus Hostuviroid. These pathogens are distributed worldwide and infect a large number of hosts. In Brazil, isolates of CEVd and HSVd have been detected in both citrus and grapevine. To characterize and study the genetic variability of these viroids, total RNA from leaves of grapevine Vitis vinifera 'Cabernet Sauvignon' and V. labrusca 'Niagara Rosada' from Bento Gonçalves, RS, was used as a template for RT-PCR amplification with specific primers for the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: CEVd; HSVd; Pospiviroid; Hostuviroid; Pospiviroidae. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582006000500002 |
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