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Regua-Mangia,Adriana Hamond; Gomes,Tânia Aparecida Tardelli; Andrade,João Ramos Costa; Vieira,Mônica Aparecida Midolli; Gonzalez,Alice Gonçalves Martins; Zahner,Viviane; Irino,Kinue; Teixeira,Lúcia Martins. |
In the present study, 47 enteropathogenic Escherichia coli strains identified according to serotyping, presence of eae, bfp and EAF sequences, adherence phenotype and ability to induce attaching-effacing lesions were analyzed by pulsed-field gel electrophoresis (PFGE), multilocus enzyme electrophoresis (MLEE), and the presence of LEE genes (eae, espA, espB, tir) as well as the respective alleles. Amplification of LEE genes subtypes revealed 18 different pathotypes. Typing of the eae gene showed that most strains contained nontypable intimin (42%) followed by beta (35%), gamma and alpha genes (12% each). PFGE analysis revealed a variable degree of polymorphism among isolates and, in general, no clear correlation was observed among PFGE profiles and the... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Enteropathogenic Escherichia coli; Genetic diversity; Virulence markers. |
Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500013 |
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d'Azevedo,Pedro Alves; Dias,Cícero Armídio Gomes; Lemos,Sibele Krebs; Bittencourt,José Augusto Ferreira; Teixeira,Lúcia Martins. |
Resistance to several classes of antimicrobial agents is a remarkable characteristic of enterococcal strains increasingly reported worldwide. Information about strains isolated in the southern region of Brazil is still limited. In this study, a total of 455 consecutive enterococcal isolates recovered from patients living in Porto Alegre, Brazil, were identified at species level and evaluated for their antimicrobial susceptibilities by agar diffusion testing. The most frequent species was E. faecalis (92.8%), followed by E. faecium (2.9%), E. gallinarum (1.5%), E. avium (1.1%), E. hirae (0.7%), E. casseliflavus (0.4%), E. durans (0.4%), and E. raffinosus (0.2%). According to the results of disk tests 62.0% of the strains were resistant to tetracycline,... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Enterococcal; Antimicrobial susceptibility; Resistance. |
Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000200005 |
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