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A broad pH range and processive chitinase from a metagenome library BJMBR
Thimoteo,S.S.; Glogauer,A.; Faoro,H.; de Souza,E.M.; Huergo,L.F.; Moerschbacher,B.M.; Pedrosa,F.O..
Chitinases are hydrolases that degrade chitin, a polymer of N-acetylglucosamine linked β(1-4) present in the exoskeleton of crustaceans, insects, nematodes and fungal cell walls. A metagenome fosmid library from a wastewater-contaminated soil was functionally screened for chitinase activity leading to the isolation and identification of a chitinase gene named metachi18A. The metachi18A gene was subcloned and overexpressed in Escherichia coli BL21 and the MetaChi18A chitinase was purified by affinity chromatography as a 6xHis-tagged fusion protein. The MetaChi18A enzyme is a 92-kDa protein with a conserved active site domain of glycosyl hydrolases family 18. It hydrolyses colloidal chitin with an optimum pH of 5 and temperature of 50°C. Moreover, the enzyme...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chitinase; Metagenomic; Aeromonas; Kinetics.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000100602
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Occurrence of Candida orthopsilosis in Brazilian tomato fruits (Lycopersicum esculentum Mill.) BJM
Robl,D.; Thimoteo,S.S.; Souza,G.C.C.F. de; Beux,M.R.; Dalzoto,P.R.; Pinheiro,R.L.; Pimentel,I.C..
We aimed to isolate and identify yeasts found in the tomato fruit in order to obtain isolates with biotechnological potential, such as in control of fungal diseases that damage postharvest fruits. We identified Candida orthopsilosis strains LT18 and LT24. This is the first report of this yeast on Lycopersicum esculentum fruits in Brazil.
Tipo: Info:eu-repo/semantics/other Palavras-chave: Lycopersicum esculentum Mill; Candida orthopsilosis; Tomato fruits.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100015
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