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Changes in N2 fixation in Stylosanthes scabra derived from tissue culture Braz. J. Genet.
Valarini,Maria José; Otsuk,Ivani Pozar; Vieira,Maria Lucia Carneiro.
Plants were regenerated from leaf-derived callus culture of Stylosanthes scabra, a polyploid legume tolerant to drought and adapted to acid soils. A total of 168 regenerants were planted out in Leonard jars in a complete randomized design. Nitrogen fixation and vegetative growth were indirectly evaluated by shoot dry weight, root dry weight, shoot N content and acetylene reduction activity. The results showed higher variation in the regenerants than in controls not submitted to tissue culture. Significant differences were found for all nitrogen fixation related-traits
Tipo: Info:eu-repo/semantics/other
Ano: 1997 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84551997000400025
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Plant regeneration from proroplasts of alfalfa (Medicago sativa) via somatic embryogenesis Scientia Agricola
Monteiro,Mariza; Appezzato-da-Glória,Beatriz; Valarini,Maria José; Oliveira,Carlos Alberto de; Vieira,Maria Lucia Carneiro.
Alfalfa is one of the most frequently studied species from the production of tissue culture-derived embryos point of view. In this study, five alfalfa cultivars were analyzed with reference to their ability to regenerate plants from protoplast cultures via somatic embryogenesis. Plant regeneration from leaf-derived protoplasts isolated from the cultivar Rangelander was achieved using a protocol defined for alfalfa cell suspension-derived embryogenesis. Because of its high efficiency, this procedure is recommended for protoplast electroporation-mediated genetic transformation of alfalfa.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Plant biotechnology; In vitro culture; Morphogenesis; Forage legume.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162003000400012
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