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| Vedrine, C.; Lazerges, M.; Perrot, H.; Compere, Chantal; Pernelle, C.. |
| A strategy to design DNA-biosensors by grafting probe-target duplex onto a 27 MHz quartz crystal microbalance gold surface is presented in this work. The idea that removing the DNA target after duplex grafting yields to a well accessible probe, and therefore to efficient target recognition, is investigated. Unfortunately, hybridization efficiency measured using such a straightaway protocol is equal to 15%, which is feeble by comparison with the 31% hybridization efficiency measured for biosensors designed with single-stranded probe. Successive DNA biosensors are designed in this work, taking into account phenomena that occur specifically in an interfacial environment, like non specific adsorption or exchange reactions between adsorbed molecules. A... |
| Tipo: Text |
Palavras-chave: Alexandrium minutum; Biosensor; Double-stranded DNA; Quartz crystal microbalance. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00101/21196/19416.pdf |
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