|
|
|
|
|
| Rojas,Mariana; Venegas,Felipe; Montiel,Enrique; Servely,Jean Luc; Vignon,Xavier; Guillomot,Michel. |
| The somatic cloning by transfer of the nuclei of differentiated adult cells to previously enucleated oocytes is a promising technique for the production of embryos of high genetic value. The better mastering of somatic cloning gives us the possibility to produce embryos from endangered species. The huemul is an Andean native deer, that has been declared an endangered species, it holds a great patrimonial value and it is a Chilean national emblem. In Chile the huemul has the status of protected animal on thirteen Parks and National Reserves managed by Corporacion Nacional Forestal (CONAF). This protection, however, is considered insufficient due to the few geographical connections between the different protected areas. Furthermore, a great proportion of... |
| Tipo: Journal article |
Palavras-chave: Huemul; Interspecific cloning; Endangered species. |
| Ano: 2005 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022005000400008 |
| |
|
|
| Rojas,Mariana; Venegas,Felipe; Servey,Jean Luc; Guillomot,Michel. |
| La Biología del Desarrollo y la Biotecnología han avanzado enormemente en los últimos años. Los estudios que se están realizando actualmente con nuevas técnicas moleculares, nos conducen a una nueva era en el diagnóstico y en el tratamiento de muchas enfermedades congénitas. Actualmente, se ha logrado aplicar los conocimientos derivados de ella, con incipiente éxito, en los aspectos productivos y también en la terapia de distintas enfermedades del Hombre y de otros animales y, por último, existen importantes perspectivas en la protección de especies amenazadas. En este Review, se analizan algunos de los aspectos técnicos más interesantes de la clonación, producción de quimeras y obtención de células pluripotenciales, como también los objetivos y... |
| Tipo: Journal article |
Palavras-chave: Clonación; Quimeras; Células pluripotenciales. |
| Ano: 2004 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022004000400018 |
| |
|
|
| Venegas,Felipe; Guillomot,Michel; Vignon,Xavier; Servely,Jean-Luc; Audouard,Chistophe; Montiel,Enrique; Le Bourhis,Daniel; Perón,Sandrine; Soto,Pilar; Rojas,Mariana. |
| The objective of this project is to produce pudu embryos, obtained by means of fibroblast transfer taken from the ear of one animal, to the oocytes of a domestic ruminant such as cattle. In further work, the pudu embryos will be transferred to the uterus of synchronized recipient females of another species. Biopsies 1mm in size were obtained from the outer rim of the ear of two male pudu deer belonging to the Buin Zoological park, Santiago, Chile. The cell lines have been established and kept according to protocols used in cattle. The oocytes are obtained through a puncture in the oocyte-cumulus complex (OCC) from cow ovaries recovered from the butcher. Each oocyte is enucleated and fused with an isolated fibroblast which is inserted beneath the pellucid... |
| Tipo: Journal article |
Palavras-chave: Pudu deer; Cervidae; Ruminant; Cloning. |
| Ano: 2006 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022006000300026 |
| |
|
|
| Venegas,Felipe; Montiel,Enrique; Forno,Pablo; Rojas,Mariana. |
| In adult salmon of the sea centres in southern Chile, a jaw deformation (JD) has been identified. It affects the dental and hyomandibular bones, which bend ventrally up to 90° of their normal position. The deformation affects also the dental articular bone. This pathology is related to weight loss and increased mortality of the salmons. It was empirically postulated that a probable cause for this anomaly was food from vegetal origin in the diet of the fishes (which are carnivores) Therefore, the present work aims at comparing the biostructure of jaw bone of salmons fed either with vegetal (soja and gluten) formulation or animal formulation, mostly fish powder. Fifty specimens were analyzed from Puerto Montt, 35 having JD and 15 normal control.... |
| Tipo: Journal article |
Palavras-chave: Jaw; Jaw deformation; Histology; Salmon; Salmo salar. |
| Ano: 2003 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022003000300005 |
| |
|
|
| Illanes,Julio; Orellana,Cupertina; Fertilio,Bárbara; Leyton,Victor; Venegas,Felipe. |
| La gata doméstica (Felis catus) presenta una gestación que dura, en promedio 62 + 5 días. Sin embargo, establecer la edad gestacional en forma más o menos precisa resulta difícil, ya que no existe un análisis que correlacione el tamaño de la vesícula coriónica; desarrollo de la placenta; desarrollo embrionario y Fetal durante la gestación. En este trabajo se utilizaron 12 úteros grávidos, provenientes de gatas mestizas entre 8 y 18 meses de edad, éstos fueron fijados en formol neutro al 10%. Para cada útero se determinó el número de vesículas coriónicas, de cada una se removieron el embrión, feto y la placenta, los que fueron medidos, tarados y fotografiados para su análisis morfológico. Las etapas del desarrollo embrionario y Fetal fueron establecidas... |
| Tipo: Journal article |
Palavras-chave: Gato; Felis catus; Embriología; Desarrollo embrionario; Desarrollo Fetal. |
| Ano: 2007 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022007000300002 |
| |
|
|
|
