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Hernández,P.J.E; Fernández,R.F; Rodríguez,S.J.L; Soto,M.Y.G; Verona,J.E.H; García,R.A.D. |
In order to evaluate the post-thaw effect on acrosomal viability and reaction of sperm collected from equine epididymis tail, 62 testes were collected and transported at 4°C. Epididymis was dissected to perform the retrograde flushing of the epididymis tail , using 10 mL of a diluent based on skim milk (INRA 82). The diluent obtained with the spermatic content was recovered in graduated collecting tubes and 1mL of each sample was taken to be evaluated. For evaluations, the samples having a minimum of 30% of progressive motility were selected. The evaluations were on: percentage of live sperm, normal morphology, sperm concentration, percentage of live sperm without acrosomal reaction (VsRA) and live with acrosomal reaction (VcRA), evaluating this indicator... |
Tipo: Journal article |
Palavras-chave: Cryopreservation; Viability; Acrosomal reaction; FITC-PNA/IP; Sperm; Epididymis; Equine. |
Ano: 2012 |
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2012000200004 |
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