|
|
|
|
| |
|
|
| Moreira,Mônica; Adamoski,Douglas; Sun,Jiufeng; Najafzadeh,Mohammad Javad; Nascimento,Mariana Machado Fidelis do; Gomes,Renata Rodrigues; Barbieri,Dicler de Sant'Anna; Glienke,Chirlei; Klisiowicz,Débora do Rocio; Vicente,Vânia Aparecida. |
| The aim of this study was to develop and evaluate a padlock probe based on the Rolling Circle Amplification (RCA), which targeted to 16S-23S rDNA region of S. mutans. The specificity of developed padlock probe was tested for DNA within a panel strains, including S. mutans isolated from the saliva and reference strains of the genus Streptococcus, as well as total DNA samples of biofilm and saliva. The results were positive either for DNA samples of S. mutans or DNA samples recovered from the biofilm and saliva revealing the specificity of designed padlock probe. The padlock probe based on the RCA was proved to be an effective, reproducible method for S. mutansdetection and demonstrated the possibility of a rapid detection and accurate identification of S.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Padlock probe; RCA; Streptococcus mutans. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000100054 |
| |
|
| |
|
|
| Marangoni,Paulo Roberto Dantas; Robl,Diogo; Berton,Marcos Antonio Coelho; Garcia,Carlos Mario; Bozza,Angela; Porsani,Mariana Vieira; Dalzoto,Patricia do Rocio; Vicente,Vânia Aparecida; Pimentel,Ida Chapaval. |
| The aim of this study was evaluate, two methods for the detection and identification of sulphate reducing bacteria (SRB): ML medium and PCR with specific primers for SRB groups. SRB were detected through the selective medium only on carbon steel, which showed corrosion. Employing specific PCR primer, SBR were detected from all the metallic components assayed, even those that did not present visible corrosion spots, such stainless steel and copper alloys. Despite the presence or absence of corrosion at the later stages effectively by using the selective medium,, the initial stages of the corrosion could only be detected by the amplification of total DNA with SRB specific primers. The early detection of SRB could be employed for preventing the damages on... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Metallic surface; Hydroelectric power station; Biofilm; Biocorrosion. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000500011 |
| |
|
| |
|
|
| Vicente,Vânia Aparecida; Angelis,Derlene Attili de; Queiróz-Telles Filho,Flávio; Pizzirani-Kleiner,Aline Aparecida. |
| Herpotrichiellaceous fungi, common agents of chromoblastomycosis and phaeohyphomycosis, were searched in samples of rotten wood, leaf littler, bark and soil of the rhizosphere, collected in the Centro Nacional de Pesquisas de Florestas/EMBRAPA, Colombo, PR, Brazil. Morphological analyses of macro, optic and scanning electron microscopy, as well as the determination of the nutritional pattern of the isolated strains were carried out for a taxonomical study. In a total of 17 colonies, 3 isolates (17.6%) were identified as species of medical relevance: Cladophialophora bantiana (Sacc.) de Hoog et al., Fonsecaea pedrosoi (Brumpt) Negroni and Phialophora verrucosa Medlar. The identifications were based on the results of the analyses and on the comparison with... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chromoblastomycosis; Fungal isolation; Herpotrichiellaceae; Phaeohyphomycosis. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000100011 |
| |
|
| |
|
|
|
