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Vidotto,Marilda C.; Lima,Natália C.S. de; Fritzen,Juliana T.T.; Freitas,Júlio C. de; Venâncio,Emerson J.; Ono,Mario A.. |
Identification of Escherichia coli causing porcine postweaning diarrhea requires knowledge regarding the prevalent pathotypes within a given region. A total of 100Escherichia coli isolates from piglets with diarrhea in Londrina city, Parana State, South Brazil, were screened for the presence of genes for F4, F5, F6, F18, F41 fimbrial antigens by specific probes and for enterotoxins (STa, STb, LT and STx2e) by polymerase chain reaction (PCR). The results showed that 60% of the isolates were positive for one or more of the fimbrial antigens and 92% were positive at least for one of the virulence factors examined. Virulence factor genes detected were F4 (44%), F18 (38%), F5 (30%), F41 (32%), F6 (25%), LTp-I (71%), STa (40%), STb (47%) and STx2e (3%). Twenty... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Porcine Escherichia coli; Virulence genes; Postweaning diarrhea; Toxins. |
Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000100035 |
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Kobayashi,Renata K. T.; Saridakis,Halha Ostrensky; Dias,Angela M.G.; Vidotto,Marilda C.. |
In this work, the prevalence of enteropathogenic Escherichia coli (EPEC) in children in Londrina-PR, Brazil, was evaluated by means of digoxigenin-labelled DNA probes which identify the plasmid responsible for EPEC adherence factor (EAF), and virulence genes for EPEC as bundle-forming pilus (bfp) and E. coli attaching-effacing factor (eae). In addition, the isolated strains were serotyped and tested for adherence to HEp-2 cells. From 102 children with diarrhoea, 19 strains hybridized with at least one probe, and eleven of them were identified as typical EPEC because they hybridized with the three probes used, showed a localized adherence (LA) pattern, and presented no genes for enterotoxins (ST and LT) or invasion as detected by PCR. Six of the typical... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Enteropathogenic Escherichia coli; EPEC; Digoxigenin-labelled DNA probes; Virulence factors; Serotypes; Diarrhoea. |
Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000400007 |
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