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Attempts at Applying Cloning to the Conservation of Species in Danger of Extinction International Journal of Morphology
Rojas,Mariana; Venegas,Felipe; Montiel,Enrique; Servely,Jean Luc; Vignon,Xavier; Guillomot,Michel.
The somatic cloning by transfer of the nuclei of differentiated adult cells to previously enucleated oocytes is a promising technique for the production of embryos of high genetic value. The better mastering of somatic cloning gives us the possibility to produce embryos from endangered species. The huemul is an Andean native deer, that has been declared an endangered species, it holds a great patrimonial value and it is a Chilean national emblem. In Chile the huemul has the status of protected animal on thirteen Parks and National Reserves managed by Corporacion Nacional Forestal (CONAF). This protection, however, is considered insufficient due to the few geographical connections between the different protected areas. Furthermore, a great proportion of...
Tipo: Journal article Palavras-chave: Huemul; Interspecific cloning; Endangered species.
Ano: 2005 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022005000400008
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Obtaiment of Pudu (Pudu pudu) deer Embryos by the Somatic Nuclear Transfer Technique International Journal of Morphology
Venegas,Felipe; Guillomot,Michel; Vignon,Xavier; Servely,Jean-Luc; Audouard,Chistophe; Montiel,Enrique; Le Bourhis,Daniel; Perón,Sandrine; Soto,Pilar; Rojas,Mariana.
The objective of this project is to produce pudu embryos, obtained by means of fibroblast transfer taken from the ear of one animal, to the oocytes of a domestic ruminant such as cattle. In further work, the pudu embryos will be transferred to the uterus of synchronized recipient females of another species. Biopsies 1mm in size were obtained from the outer rim of the ear of two male pudu deer belonging to the Buin Zoological park, Santiago, Chile. The cell lines have been established and kept according to protocols used in cattle. The oocytes are obtained through a puncture in the oocyte-cumulus complex (OCC) from cow ovaries recovered from the butcher. Each oocyte is enucleated and fused with an isolated fibroblast which is inserted beneath the pellucid...
Tipo: Journal article Palavras-chave: Pudu deer; Cervidae; Ruminant; Cloning.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022006000300026
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