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| Antonello,A. M.; Pivoto,F. L.; Camillo,G.; Braunig,P.; Sangioni,L. A.; Pompermayer,E.; Venturini,M. C.; Vogel,F.S.F.. |
| ABSTRACTSarcocystis neurona is the primary agent for Equine Protozoal Myeloencephalitis (EPM), important neurological disease characterized by behavior or muscular changes, that impairs animal performance and husbandry. Sarcocystis cruzi is a pathogen related to myositis in cattle. Although related the life cycles of the parasites are distinct. S. neurona has opossums (Didelphis spp.) and S. cruzi, dogs as definitive hosts. However, S. neurona and S. cruzi may undergo cross-reactivity in serological tests, interfering on results of EPM ante-mortem diagnostic tests. In the present study, serology of 189 mares was performed by indirect immunofluorescence antibody test, using antigens of S. neurona and S. cruzi in order to assess the exposure degree of... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Sorologia; Soroprevalência; Mieloencefalite equina por protozoário; Apicomplexa. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000501465 |
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| Antonello,A.M.; Cadore,G.C.; Pivoto,F.L.; Camillo,G.; Braunig,P.; Sangioni,L.A.; Pompermayer,E.; Gondim,L.F.P.; Vogel,F.S.F.. |
| The aim of this study was to examine the intra-uterine exposure to Sarcocystis spp. antigens, determining the number of foals with detectable concentrations of antibodies against these agents in the serum, before colostrum ingestion and collect data about exposure of horses to the parasite. Serum samples were collected from 195 thoroughbred mares and their newborns in two farms from southern Brazil. Parasite specific antibody responses to Sarcocystis antigens were detected using the indirect immunofluorescent antibody test (IFAT) and immunoblot analysis. In 84.1% (159/189) of the pregnant mares and in 7.4% (14/189) of foals we detected antibodies anti-Sarcocystis spp. by IFAT. All samples seropositive from foals were also positive in their respective... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Equine protozoal mieloencephalitis; Transplacental infection; Seroprevalence; Indirect fluorescent antibody test; Immunoblot. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000200271 |
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| Mayer,S.V.; de Quadros,V.L.; Vogel,F.S.F.; Winkelmann,E.R.; Arenhart,S.; Weiblen,R.; Flores,E.F.. |
| Bovine herpesvirus type 5 (BHV-5) is a major agent of meningoencephalitis in cattle and establishes latent infections mainly in sensory nerve ganglia. The distribution of latent BHV-5 DNA in the brain of rabbits prior to and after virus reactivation was studied using a nested PCR. Fifteen rabbits inoculated intranasally with BHV-5 were euthanized 60 days post-inoculation (group A, N = 8) or submitted to dexamethasone treatment (2.6 mg kg-1 day-1, im, for 5 days) and euthanized 60 days later (group B, N = 7) for tissue examination. Two groups of BHV-1-infected rabbits (C, N = 3 and D, N = 3) submitted to each treatment were used as controls. Viral DNA of group A rabbits was consistently detected in trigeminal ganglia (8/8), frequently in cerebellum (5/8),... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovine herpesvirus type 5; BHV-5; BHV-1; Latent infection; Rabbits; Brain. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000300004 |
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| Quadros,V.L.; Mayer,S.V.; Vogel,F.S.F.; Weiblen,R.; Brum,M.C.S.; Arenhart,S.; Flores,E.F.. |
| Calves born persistently infected with non-cytopathic bovine viral diarrhea virus (ncpBVDV) frequently develop a fatal gastroenteric illness called mucosal disease. Both the original virus (ncpBVDV) and an antigenically identical but cytopathic virus (cpBVDV) can be isolated from animals affected by mucosal disease. Cytopathic BVDVs originate from their ncp counterparts by diverse genetic mechanisms, all leading to the expression of the non-structural polypeptide NS3 as a discrete protein. In contrast, ncpBVDVs express only the large precursor polypeptide, NS2-3, which contains the NS3 sequence within its carboxy-terminal half. We report here the investigation of the mechanism leading to NS3 expression in 41 cpBVDV isolates. An RT-PCR strategy was employed... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovine viral diarrhea virus; CpBVDV; NS3 gene; Cytopathology; RNA processing. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000700012 |
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