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| Pereira,Daniela Isabel Brayer; Botton,Sônia de Avila; Azevedo,Maria Isabel de; Monteiro,Danieli Urach; Weiblen,Carla; Machado,Vanessa Schopf; Donatti,Michele Guimarães; Loeck,Alci Enimar. |
| Leaf-cutting ants of the genera Atta and Acromyrmex determine serious agricultural problems and live on symbiosis with Leucoagaricus gongylophorus. The aim of this study is to identify morphological and molecularly, as well as to verify the genotypic variability of the symbiotic fungus cultivated by A. heyeri and A. ambiguus from three different regions of the state of Rio Grande do Sul, Brazil. Fungus gardens were collected and fragments of mycelia were grown in selective medium. Total DNA was extracted and amplification of the ITS region was performed by PCR using universal primers. After DNA sequencing, the chromatograms were assembled and phylogenetic analyzes were performed by the Neighbor-Joining method. A total of six isolates of L. gongylophorus... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Basidiomycete; Leucoagaricus gongylophorus; Internal transcribed spacer.. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000701256 |
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| Weiblen,Carla; Azevedo,Maria Isabel de; Ianiski,Lara Baccarin; Stibbe,Paula Cristina; Pereira,Daniela Isabel Brayer; Zanette,Régis Adriel; Sangioni,Luís Antônio; Rivero,Rodolfo; Santurio,Janio Morais; Botton,Sônia de Avila. |
| ABSTRACT: We aimed to genotype the South American clinical isolates of Pythium insidiosum using the single nucleotide polymorphisms (SNP) of the ribosomal DNA sequences (rDNA). Previously, an SNP-based multiplex-PCR was able to distinguish three different clades of P. insidiosum isolates. Thus, we used this assay to evaluate South American clinical isolates of P. insidiosum (n=32), standard strains from Costa Rica (n=4), Thailand (n=3), Japan (n=1), and India (n=1), a standard strain of Pythium aphanidermatum, and Brazilian environmental isolates of Pythium torulosum, Pythium rhizo-oryzae and Pythium pachycaule voucher (n=3). It was possible to allocate each American P. insidiosum isolate to clade I, the isolates of India, Japan, and Thailand to clade II,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pythium insidiosum; Pythiosis; Molecular detection; Genotype; Single nucleotide polimorphisms. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000100450 |
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| Weiblen,Carla; Machado,Gustavo; Jesus,Francielli Pantella Kunz de; Santurio,Janio Morais; Zanette,Régis Adriel; Pereira,Daniela Sabel Brayer; Diehl,Gustavo Nogueira; Santos,Lucila Carboneiro dos; Corbellini,Luis Gustavo; Botton,Sônia de Avila. |
| ABSTRACT: An epidemiological survey was carried out by performing an Enzyme Linked Immuno Sorbent Assay (ELISA) test to determine the seroprevalence of Pythium insidiosum infection in equine in Rio Grande do Sul State (RS), Brazil. The serological study covered seven geographical regions of RS, classified according to the Instituto Brasileiro de Geografia e Estatística (IBGE). The samples were obtained from official veterinary service (Serviço Veterinário Oficial, SVO) linked to the Secretaria da Agricultura, Pecuária e Agronegócio of RS (SEAPA-RS) to proceed the investigation of equine infectious anemia in 2014. Samples were collected during the months of September and October of 2013, covering the seven geographical regions of RS, and totalized 1,002... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: P. insidiosum; Antibody; Occurrence; Horses; ELISA. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000100126 |
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| Weiblen,Carla; Pereira,Daniela Isabel Brayer; Dutra,Valéria; Godoy,Isabela de; Nakazato,Luciano; Sangioni,Luís Antonio; Santurio,Janio Morais; Botton,Sônia de Avila. |
| ABSTRACT: Conidiobolomycosis is an emerging disease caused by fungi of the cosmopolitan genus Conidiobolus . Particular strains of Conidiobolus coronatus, Conidiobolus incongruus and Conidiobolus lamprauges , mainly from tropical or sub-tropical origin, cause the mycosis in humans and animals, domestic or wild. Lesions are usually granulomatous and necrotic in character, presenting two clinical forms: rhinofacial and nasopharyngeal. This review includes the main features of the disease in sheep, with an emphasis on the epidemiology, clinical aspects, and diagnosis of infections caused by Conidiobolus spp. in Brazil. In this country, the disease is endemic in the Northeast and Midwest, affecting predominantly woolless sheep breeds and occasioning death... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fungal infection; Ovine; Zygomycosis; Conidiobolus spp .. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000500839 |
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