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| Homrich,Milena Schenkel; Wiebke-Strohm,Beatriz; Weber,Ricardo Luís Mayer; Bodanese-Zanettini,Maria Helena. |
| Transgenic plants represent an invaluable tool for molecular, genetic, biochemical and physiological studies by gene overexpression or silencing, transposon-based mutagenesis, protein sub-cellular localization and/or promoter characterization as well as a breakthrough for breeding programs, allowing the production of novel and genetically diverse genotypes. However, the stable transformation of soybean cannot yet be considered to be routine because it depends on the ability to combine efficient transformation and regeneration techniques. Two methods have been used with relative success to produce completely and stably transformed plants: particle bombardment and the Agrobacterium tumefaciens system. In addition, transformation by Agrobacterium rhizogenes... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Transgenic plants; Particle bombardment; Agrobacterium systems; Functional analysis; Genetic improvement. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000600015 |
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| Osorio,Marina Borges; Bücker-Neto,Lauro; Castilhos,Graciela; Turchetto-Zolet,Andreia Carina; Wiebke-Strohm,Beatriz; Bodanese-Zanettini,Maria Helena; Margis-Pinheiro,Márcia. |
| Environmental stresses caused by either abiotic or biotic factors greatly affect agriculture. As for soybean [Glycine max (L.) Merril], one of the most important crop species in the world, the situation is not different. In order to deal with these stresses, plants have evolved a variety of sophisticated molecular mechanisms, to which the transcriptional regulation of target-genes by transcription factors is crucial. Even though the involvement of several transcription factor families has been widely reported in stress response, there still is a lot to be uncovered, especially in soybean. Therefore, the objective of this study was to investigate the role of bHLH and trihelix-GT transcription factors in soybean responses to environmental stresses. Gene... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Drought; Gene expression; Glycine max; Phylogeny; Plant-microbe interactions. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000200005 |
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| Rechenmacher,Ciliana; Wiebke-Strohm,Beatriz; Oliveira-Busatto,Luisa A. de; Polacco,Joseph C.; Carlini,Célia R.; Bodanese-Zanettini,Maria H.. |
| Abstract Urease catalyzes the hydrolysis of urea to ammonia and carbon dioxide. The ammonia (nitrogen (N) product of urease activity) is incorporated into organic compounds. Thus, urease is involved in N remobilization, as well as in primary N assimilation. Two urease isoforms have been described for soybean: the embryo-specific, encoded by the Eu1 gene, and the ubiquitous urease, encoded by Eu4. A third urease-encoding gene was recently identified, designated Eu5, which encodes the putative protein product SBU-III. The present study aimed to evaluate the contribution of soybean ureases to seed germination and plant development. Analyses were performed using Eu1/Eu4/Eu5-co-suppressed transgenic plants and mutants of the Eu1 and Eu4 urease structural genes,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Plant development; Functional study; Transgenic plants; Mutants; Urease. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000200209 |
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| Bücker Neto,Lauro; Oliveira,Rafael Rodrigues de; Wiebke-Strohm,Beatriz; Bencke,Marta; Weber,Ricardo Luís Mayer; Cabreira,Caroline; Abdelnoor,Ricardo Vilela; Marcelino,Francismar Correa; Zanettini,Maria Helena Bodanese; Passaglia,Luciane Maria Pereira. |
| Soybean [Glycine max (L.) Merril], one of the most important crop species in the world, is very susceptible to abiotic and biotic stress. Soybean plants have developed a variety of molecular mechanisms that help them survive stressful conditions. Hybrid proline-rich proteins (HyPRPs) constitute a family of cell-wall proteins with a variable N-terminal domain and conserved C-terminal domain that is phylogenetically related to non-specific lipid transfer proteins. Members of the HyPRP family are involved in basic cellular processes and their expression and activity are modulated by environmental factors. In this study, microarray analysis and real time RT-qPCR were used to identify putative HyPRP genes in the soybean genome and to assess their expression in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fungal disease; HyPRP genes; Glycine max; Real time RT-qPCR. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000200012 |
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| Droste,Annette; Silva,Anelise Machado da; Souza,Iliane Freitas de; Wiebke-Strohm,Beatriz; Bücker-Neto,Lauro; Bencke,Marta; Sauner,Márcia Viviane; Bodanese-Zanettini,Maria Helena. |
| The aim of this work was to identify Brazilian soybean (Glycine max) genotypes with potential to respond to in vitro culture stimuli for primary somatic embryo induction, secondary embryo proliferation and plant regeneration. Differences among eight tested cultivars were observed at each stage. Two cultivars, IAS-5 and BRSMG 68 Vencedora, were selected for the evaluation of the capacity for embryo differentiation and plant regeneration. These cultivars had high embryo induction frequencies, repetitive embryogenic proliferation, and low precocious embryo germination in the initial experiment. The effect of abscisic acid (ABA) and charcoal addition on plant regeneration was investigated. The addition of ABA to proliferation medium and of ABA and activated... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Glycine max; Brazilian cultivars; Embryogenic potential; Genetic engineering; Soybean transformation. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2010000700012 |
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