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| Registros recuperados: 53 | |
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| Mazurais, David; Ferraresso, Serena; Gatta, Pier Paolo; Desbruyeres, Elisabeth; Severe, Armelle; Corporeau, Charlotte; Claireaux, Guy; Bargelloni, Luca; Zambonino, Jose-luis. |
| Coastal systems could be affected by hypoxic events brought about by global change. These areas are essential nursery habitats for several fish species including the common sole (Solea solea L.). Tolerance of fish to hypoxia depends on species and also on their physiological condition and nutritional status. Indeed, high dietary lipid content has been recently shown to negatively impact the resistance of sole to a severe hypoxic challenge. In order to study the molecular mechanisms involved in the early response to hypoxic stress, the present work examined the hepatic transcriptome in common sole fed diets with low and high lipid content, exposed to severe hypoxia. The activity of AMP-activated protein kinase (AMPK) was also investigated through the... |
| Tipo: Text |
Palavras-chave: Fish; Hypoxia; Nutrition; Liver; Transcriptome; AMPK. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00169/28062/26474.pdf |
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| Vagner, Marie; Robin, Jean; Zambonino, Jose-luis; Person, Jeannine. |
| The purpose of this study was to investigate the combined effect of the incorporation of vegetable products in diet and temperature on enzymatic pathways for high unsaturated fatty acids (HUFA) desaturation in sea bass larvae. Four replicated groups were fed a low (LH; 0.8% EPA+DHA) or a high (HH; 2.2% EPA+DHA) n-3 HUFA microparticulated diet from mouth opening, six days post-hatching and were reared at 16 or 22 degrees C. The four experimental conditions (LH16, HH16, LH22 and HH22) were tested for 45 days. At the end of the experiment, body weight, total length and biomass were affected by temperature (P<0.001), while biomass as well as fresh body weight was also influenced by diet (P<0.05 and P<0.001 respectively). This always lead to the same... |
| Tipo: Text |
Palavras-chave: Desaturation; HUFA; Dicentrarchus labrax; Delta 6 desaturase; Aquaculture. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-2673.pdf |
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| Villeneuve, Laure; Gisbert, Enric; Moriceau, Jacques; Cahu, Chantal; Zambonino, Jose-luis. |
| The effect of the feeding period on larval development was investigated in European sea bass larvae by considering the expression level of some genes involved in morphogenesis. Larvae were fed a control diet except during three different periods (period A: from 8 to 13 d post-hatching (dph); period B: from 13 to 18 dph; period C: from 18 to 23 dph) with two compound diets containing high levels of vitamin A or PUFA. European sea bass morphogenesis was affected by these two dietary nutrients during the early stages of development. The genes involved in morphogenesis could be modulated between 8 and 13 dph, and our results indicated that retinoids and fatty acids influenced two different molecular pathways that in turn implicated two different gene cascades,... |
| Tipo: Text |
Palavras-chave: Retinoid pathway; Morphogenesis; Vitamin A; PUFA; Sea bass larvae. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1215.pdf |
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| Gerard, Andre; Thouard, Emmanuel; Zambonino, Jose-luis; Gaignon, Jean-louis; Antoine, Loic; Harache, Yves; Veron, Gerard; Perez, Jose. |
| The repopulating of aquatic environments has long been based on the idea that in order to increase halieutic resources, one just had to artificially increase the number of fish in their early stages in marine or fresh water environments. Therefore, as the knowledge on the reproduction of some species and the mastery of their artificial reproduction was growing, the capacity to produce large amounts of eggs or fries from different fish or mollusc species became a reality. Controlled reproduction and the availability of large quantities of fertilized eggs, and then of larvae showing, in captivity, a greater survival capacity than naturally reproduced ones, lead to an increase of repopulating attempts through the release of fries or young fishes raised in... |
| Tipo: Text |
Palavras-chave: Recommandation; Repeuplement; Milieu aquatique; Ressources halieutiques. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/rapport-6532.pdf |
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| Zambonino, Jose-luis; Claireaux, Guy; Ernande, Bruno; Quazuguel, Patrick; Severe, Armelle; Huelvan, Christine; Mazurais, David. |
| An individual's environmental history may have delayed effects on its physiology and life history at later stages in life because of irreversible plastic responses of early ontogenesis to environmental conditions. We chose a marine fish, the common sole, as a model species to study these effects, because it inhabits shallow marine areas highly exposed to environmental changes. We tested whether temperature and trophic conditions experienced during the larval stage had delayed effects on life-history traits and resistance to hypoxia at the juvenile stage. We thus examined the combined effect of global warming and hypoxia in coastal waters, which are potential stressors to many estuarine and coastal marine fishes. Elevated temperature and better trophic... |
| Tipo: Text |
Palavras-chave: Fish; Environmental programming; Climate change; Hypoxia; Nutrition. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00127/23858/21810.pdf |
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| Cahu, Chantal; Zambonino, Jose-luis; Corraze, Genevieve; Coves, Denis. |
| Triplicate groups of juvenile seabass (initial weight of 241 g) were fed during 13 weeks three isonitrogenous experimental diets containing different lipid levels, 12% (LL group), 21% (ML group) and 30% (HL group). At the end of the experiment, fish weight gain was similar among the 3 dietary groups. Intestinal brush border membranes were purified for each dietary group; one part of the brush border fraction was dedicated to enzyme assays, the remaining fraction being used for lipid extraction followed by fatty acid analysis. The fatty acid composition of the brush border membrane differed among the 3 groups, although the 3 experimental diets had the same fatty acid composition. The increase in dietary lipid level resulted in a lowering in (n-3)... |
| Tipo: Text |
Palavras-chave: Membrane lipid; Intestine; Fish; Fatty acids; Dietary lipids; Brush border hydrolases. |
| Ano: 2000 |
URL: http://archimer.ifremer.fr/doc/2000/publication-901.pdf |
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| Vagner, Marie; Robin, Jean H.; Zambonino, Jose-luis; Tocher, Douglas R.; Person, Jeannine. |
| Four replicated groups of sea bass (Dicentrarchus labrax) larvae were fed diets containing an extra-high level of highly unsaturated fatty acids (HUFA) (XH; 3.7% EPA + DHA), a high level of HUFA (HH; 1.7%), a low level of HUFA (LH: 0.7%) or an extra-low level of HUFA (XLH; 0.5%) from day 6 to day 45 (experiment 1; XH1, HH1, LH1, XLH1). After a subsequent I-month period feeding a commercial diet (2.7% EPA + DRA), the capacity of the four initial groups to adapt to an n-3 HUFA-restricted diet (0-3% EPA + DHA; R-groups: XH2(R), HH2(R), LH2R, XLH2(R)) was tested for 35 d. Larval dietary treatments had no effect on larval and juvenile survival rates. The wet weight of day 45 larvae was higher in XH1 and HH1 (P<0.001), but the R-juvenile mass gains were... |
| Tipo: Text |
Palavras-chave: Programming concept; Dicentrarchus labrax; Delta 6 Desaturase; Aquaculture. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-7477.pdf |
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| Cahu, Chantal; Ronnestad, I; Grangier, V; Zambonino, Jose-luis. |
| In order to assess the influence of dietary protein on digestive enzyme expression and cholecystokinin (CCK) content in sea bass larvae, four groups of larvae were fed experimental diets from mouth opening until day 42: three isonitrogenous diets with increasing protein hydrolysate levels (0%, 14% and 46% of crude matter) and one diet incorporating starch. The groups fed high starch or high protein hydrolysate level exhibited the lowest growth. The final weight in these groups was 9.5 and 5.6 mg, respectively, whereas it reached approximately 20.0 mg in the groups fed 0% or 14% protein hydrolysate level. The highest levels of trypsin secretion were observed in larvae fed the lowest protein hydrolysate level. Paradoxically, the groups fed diet containing... |
| Tipo: Text |
Palavras-chave: Starch; Sea bass larvae; Protein hydrolysate; Pancreatic enzyme secretion; Cholecystokinin. |
| Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/2004/publication-442.pdf |
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| Santigosa, Ester; Geay, Florian; Tonon, Thierry; Le Delliou, Herve; Kuhl, Heiner; Reinhardt, Richard; Corcos, Laurent; Cahu, Chantal; Zambonino, Jose-luis; Mazurais, David. |
| Fish are the main source of the n-3 highly unsaturated fatty acids, which are crucial for human health. Their synthesis from C18 precursors is mediated by desaturases and elongases, but the activity of these enzymes has not been conclusively established in marine fish species. This study reports the cloning, tissue expression, and functional characterization of a sea bass (Dicentrarchus labrax L.) Δ6-desaturase and one of its splicing variants. Two cDNAs with open reading frames of 1,346 and 1,354 bp were cloned and named D6D and D6D-V, respectively. Both deduced protein sequences (445 and 387 amino acids, respectively) contained two transmembrane regions and the N-terminal cytochrome b5 domain with the HPGG motif characteristic of microsomal desaturases.... |
| Tipo: Text |
Palavras-chave: Sea bass (Dicentrarchus labrax); Desaturase; HUFA biosynthesis; Fish; EPA. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00023/13470/10477.pdf |
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| Fernandez, I; Hontoria, F; Ortiz Delgado, J; Kotzamanis, Y; Estevez, A; Zambonino, Jose-luis; Gisbert, E. |
| Several nutritional studies have found a direct effect of several vitamins in chondrogenic and osteogenic development during early life stages of marine fish species. In the present study, the effect of vitamin A (VA) in gilthead sea bream skeletogenesis was evaluated by means of four different dietary regimes (enriched rotifers) containing increasing levels of total VA (75,109, 188 and 723 ng total VA mg(-1) DW). Dietary treatments were offered to larvae during the rotifer-feeding phase (4-20 days after hatching), while later all groups were fed with Artemia nauplii and weaned onto the same inert diet. Different dietary doses of VA affected gilthead sea bream larval growth, survival, performance (maturation of the digestive system) and quality (incidence... |
| Tipo: Text |
Palavras-chave: Deformities; Skeleton; Vitamin A; Larval quality; Sparus aurata; Gilthead sea bream. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-4937.pdf |
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| Vagner, Marie; Zambonino, Jose-luis; Robin, Jean; Person, Jeannine. |
| The purpose of this study was to check if it is possible to influence sea bass juvenile metabolism by a conditioning of larvae from day 6 post hatching to day 45 to a low or a high HUFA compound diet (LH, 0.8% EPA + DHA and HH, 2.2% EPA + DHA) when reared at 16 or 22 °C. Following a 3-month intermediate period (at 19 °C using a commercial diet), the adaptability of the 4 initial larval groups to a HUFA experimental deprived diet (0.5% EPA + DHA) were tested at 19 °C in a 60 day-experiment (d-151211). The four experimental duplicated conditions were ex-LH16 and ex-HH16 for the 2 groups previously reared at 16 °C (initial weight, 7.3 ± 0.5 g) and ex-LH22 and ex-HH22 for the 2 groups previously reared at 22 °C (initial weight, 11.1 ± 0.5 g). Survival was... |
| Tipo: Text |
Palavras-chave: Programming concept; HUFA; Dicentrarchus labrax; Delta 6 desaturase; Aquaculture. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-2692.pdf |
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| Zambonino, Jose-luis; Cahu, Chantal. |
| During these last 20 years, many studies have focussed on the development of the digestive tract in marine fish larvae. Most of the studies aimed at acquiring knowledge on the optimal form of dietary supply for different nutrients, in order to formulate a compound diet able to totally replace live preys in the fish larvae feeding sequence. Consequently, most of the studies aimed at describing the effect of dietary adaptation on digestive enzymes profile, the morphology of the main organs, while others aspects of the physiology of the larvae digestive tract (gut hormones, intestinal transport...) were poorly investigated. This review reports the more recent data on dietary modulation of digestive enzymes with a particular emphasis, when possible, on the... |
| Tipo: Text |
Palavras-chave: Nuclear receptors; Digestive enzymes; Fatty acids; Vitamins; Dietary proteins and lipids; Fish larvae. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-3045.pdf |
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| Cahu, Chantal; Gisbert, Enric; Villeneuve, Laure A. N.; Morais, Sofia; Hamza, Neila; Wold, Per-avid; Zambonino, Jose-luis. |
| The aim of this paper is to provide explanations of how dietary phospholipid (PL) globally improves fish larval development, including growth and survival, digestive functions and skeletal development, and to propose optimal PL levels and sources in fish larval diets. Dietary incorporation of 8-12% PL related to dry matter (d.m.) promotes growth and enhanced survival in various species. Marine source PL, incorporating highly unsaturated fatty acids, was most efficient than soybean lecithin. This beneficial effect was explained by an enhancement in digestive functions, assessed by digestive enzyme activities and histomorphology. Nevertheless, 1.5-2.5% highly unsaturated fatty acids related to diet d.m. supplied by PL improved growth, survival and skeletal... |
| Tipo: Text |
Palavras-chave: Gene regulation; Skeletal development; Digestive functions; Fish larvae; Phospholipid. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6460.pdf |
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| Ma, Hongming; Cahu, Chantal; Zambonino, Jose-luis; Yu, Hairui; Duan, Qingyuan; Le Gall, Marie-madeleine; Mai, Kangsen. |
| Large yellow croaker is an important marine fish species for aquaculture in China. Larval rearing of this species is still based on live prey feeding. The aim of this study was to describe the onset and development of the main digestive enzymes during larval development of this species, in order to obtain essential data for the formulation of a compound diet adapted to larvae. Enzymatic assays were conducted from day I post hatching to day 40 in larvae fed the following live prey sequence: rotifers, Artemia nauplii, copepods, and then frozen copepods. Assays were done on whole larvae until day 19, then on dissected larvae and on purified brush border membranes of intestine. Pancreatic enzymes, amylase, and trypsin, showed high activities from early stages,... |
| Tipo: Text |
Palavras-chave: Trypsin; Pseudosciaena crocea; Fish larval development; Amylase; Aminopeptidase N; Alkaline phosphatase. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/publication-444.pdf |
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| Morais, Sofia; Conceicao, L; Ronnestad, I; Koven, W; Cahu, Chantal; Zambonino, Jose-luis; Dinis, M. |
| A growth depressing effect of high dietary neutral lipid levels in marine fish larvae has been reported. This may be a result of a decrease in the efficiency or activity of digestive enzymes, a reduction in absorption efficiency and/or a decrease in food intake. The present work reviews recent studies carried out on commercially valuable species (Atlantic herring, Senegalese sole, European seabass and gilthead seabream) that investigated the effects of neutral lipid level and lipid source (fatty acid composition) on some of these key factors influencing larval growth. The results seem to collectively indicate that lipid transport from the enterocytes into the body may be more problematic in larval stages dealing with high neutral lipid diets than lipolytic... |
| Tipo: Text |
Palavras-chave: Ingestion; Lipid droplets; Digestion; Absorption; Fatty acid composition; Lipid quantity. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-3029.pdf |
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| Zambonino, Jose-luis; Gisbert, E; Sarasquete, C; Navarro, I; Gutiérrez, J; Cahu, Chantal. |
| The ontogeny of the digestive tract of marine fish larvae has been the subject of many studies these last twenty years. Beyond the scientific interest for such animal species, particularly for developmental aspects, most of these studies aimed to come up with the expectations of commercial hatcheries by reducing the bottlenecks in larvae culture and weaning processes (switch from live preys to compound diet feeding sequence). Consequently, the profile and dietary adaptation of digestive enzymes, the description and histology of the main organs have been extensively studied, when others aspects of the physiology of the larvae digestive tract (gut hormones, intestinal transport…) were poorly investigated and this imbalanced knowledge is showed through this... |
| Tipo: Text |
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| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/00086/19684/17307.pdf |
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| Ducasse Cabanot, S; Zambonino, Jose-luis; Richard, N; Medale, Francoise; Corraze, Genevieve; Mambrini, M; Robin, Jean; Cahu, Chantal; Kaushik, Sadasivam; Panserat, S. |
| For sustainable aquaculture, the removal of marine resource ingredients in fish diets is an important objective. While most studies focus on the replacement of fish oil by vegetable oil, little is known on the nutritional effects of presence (which corresponds to the control diet) or absence of dietary fish oil. We studied fatty acid composition of brush-border membranes and digestive enzyme activities of the intestine and measured the expression and activities of several enzymes involved in the hepatic intermediary metabolism of rainbow trout (Oncorhynchus mykiss) fed for 7 weeks with or without fish oil. The diets were pair-fed to ensure that fish fed either diet had comparable carbohydrate and protein intakes. Absence of fish oil significantly reduced... |
| Tipo: Text |
Palavras-chave: Rainbow trout; Metabolism; Fish oil; Digestion; Aquaculture. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-3506.pdf |
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| Registros recuperados: 53 | |
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