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Cloning of the ω-secalin gene family in a wheat 1BL/1RS translocation line using BAC clone sequencing Electron. J. Biotechnol.
Li,Meng Jun; Li,Ya Qing; Zhang,Nan; Shi,Zhan Liang.
Background: Wheat 1BL/1RS translocation lines are planted around the world for their disease resistance and high yield. Most of them are poor in bread making, which is partially caused by ω-secalins that are encoded by the ω-secalin gene family, which is located on the short arm of rye chromosome 1R (1RS). However, information on the structure and evolution of the ω-secalin gene family is still limited. Results: We first generated a physical map of the ω-secalin gene family covering 195 kb of the Sec-1 locus based on sequencing three bacterial artificial chromosome (BAC) clones of the 1BL/1RS translocation wheat cultivar Shimai 15. A BAC contig was constructed spanning 168 kb of the Sec-1 locus on 1RS. Twelve...
Tipo: Journal article Palavras-chave: Bacterial artificial chromosome (BAC) Wheat ω -Secalin 1BL/1RS translocation line.
Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000300010
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Study and Clinical Application of the Anatomy of Renal Pedicles in Retroperitoneoscopic Nephrectomy International Journal of Morphology
Zhang,Nan; Shan,Zhongjie; Han,Qianhe; Chen,Kun; Guo,Liang.
This work aims to investigate the anatomical basis and clinical application value of renal pedicle locating in retroperitoneoscopic nephrectomy. To summarize the anatomical basis of renal pedicle locating through retrospective analysis of 278 cases of retroperitoneoscopic nephrectomy from July 2007 to September 2009, during which renal pedicle was located at about 2-4 cm below the medial arcuate ligament of the diaphragm in the space between the psoas major muscle and inferior vena cava (abdominal aorta) in the anatomical level of space before psoas. The operation of 278 patients was all successfully completed, where renal pedicle was quickly found. It took 3.5±1.3 min to locate the renal pedicle, and 95.6±23.8 min to operate. In retroperitoneoscopic...
Tipo: Journal article Palavras-chave: Laparoscopy; Nephrectomy; Anatomy; Retroperitoneoscopy.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000400001
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Ammonia determines transcriptional profile of microorganisms in anaerobic digestion BJM
Zhang,Nan; Peng,Huijuan; Li,Yong; Yang,Wenxiu; Zou,Yuneng; Duan,Huiguo.
ABSTRACT Anaerobic digestion is important for the management of livestock manure with high ammonia level. Although ammonia effects on anaerobic digestion have been comprehensively studied, the molecular mechanism underlying ammonia inhibition still remains elusive. In this study, based on metatranscriptomic analysis, the transcriptional profile of microbial community in anaerobic digestion under low (1500 mg L-1) and high NH4 + (5000 mg L-1) concentrations, respectively, were revealed. The results showed that high NH4 + concentrations significantly inhibited methane production but facilitated the accumulations of volatile fatty acids. The expression of methanogenic pathway was significantly inhibited by high NH4 + concentration but most of the other...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anaerobic digestion; Ammonia; Pathway; Gene expression; Methanogenesis.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000400770
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