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Downregulation of caffeoyl-CoA O-methyltransferase (CCoAOMT) by RNA interference leads to reduced lignin production in maize straw Genet. Mol. Biol.
Li,Xiaoyu; Chen,Wenjuan; Zhao,Yang; Xiang,Yan; Jiang,Haiyang; Zhu,Suwen; Cheng,Beijiu.
Lignin is a major cell wall component of vascular plants that provides mechanical strength and hydrophobicity to vascular vessels. However, the presence of lignin limits the effective use of crop straw in many agroindustrial processes. Here, we generated transgenic maize plants in which the expression of a lignin biosynthetic gene encoding CCoAOMT , a key enzyme involved in the lignin biosynthesis pathway was downregulated by RNA interference (RNAi). RNAi of CCoAOMT led to significantly downregulated expression of this gene in transgenic maize compared with WT plants. These transgenic plants exhibited a 22.4% decrease in Klason lignin content and a 23.3% increase in cellulose content compared with WT plants, which may reflect compensatory regulation of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: CCoAOMT; Lignin biosynthesis; Lignin and cellulose content; Maize.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000400013
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A genomic analysis of disease-resistance genes encoding nucleotide binding sites in Sorghum bicolor Genet. Mol. Biol.
Cheng,Xiao; Jiang,Haiyang; Zhao,Yang; Qian,Yexiong; Zhu,Suwen; Cheng,Beijiu.
A large set of candidate nucleotide-binding site (NBS)-encoding genes related to disease resistance was identified in the sorghum (Sorghum bicolor) genome. These resistance (R) genes were characterized based on their structural diversity, physical chromosomal location and phylogenetic relationships. Based on their N-terminal motifs and leucine-rich repeats (LRR), 50 non-regular NBS genes and 224 regular NBS genes were identified in 274 candidate NBS genes. The regular NBS genes were classified into ten types: CNL, CN, CNLX, CNX, CNXL, CXN, NX, N, NL and NLX. The vast majority (97%) of NBS genes occurred in gene clusters, indicating extensive gene duplication in the evolution of S. bicolor NBS genes. Analysis of the S. bicolor NBS phylogenetic tree revealed...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bioinformatics; Disease resistance gene; Nucleotide binding site; Sorghum bicolor.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200016
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Comparative genome analysis of the SPL gene family reveals novel evolutionary features in maize Genet. Mol. Biol.
Peng,Xiaojian; Wang,Qianqian; Zhao,Yang; Li,Xiaoyu; Ma,Qing.
Abstract SPLs are plant-specific transcription factors that play important regulatory roles in plant growth and development. Systematic analysis of the SPL family has been performed in numerous plants, such as Arabidopsis, rice, and Populus. However, no comparative analysis has been performed across different species to examine evolutionary features. In this study, we present a comparative analysis of SPLs in different species. The results showed that 84 SPLs of the four species can be divided into six groups according to phylogeny. We found that most of the SPL-containing regions in maize showed extensive conservation with duplicated regions of rice and sorghum. A gene duplication analysis in maize indicated that ZmSPLs showed a significant excess of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: SPL; Phylogenetic relationship; Gene duplication; MiR156 expression.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000300380
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