| Antagonistic activity of bacterium B. subtilis B076 on nutrient agar medium (NA) to Acidovorax avenae subsp. citrulli (Aac) isolate KK9 causal agent of bacterial fruit blotch, in the laboratory of Agricultural Biotechnology Research Center for Sustainable Economy Khon Kaen University. The results revealed that the B. subtilis B076 could exhibit the inhibition zone of diameter 1.94 cm. When cultured the bacterium antagonist in nutrient broth (NB) added with 2% glucose, the maximum ratio of heat tolerant cells production (61%) was obtained five days of incubation. The pellets of antagonistic bacteria were freeze dried by lyophilizer, the number of bacterial cell declined by 42.7%. The dust of bacterium after homogenizing with seed coating formulation... |
