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| Registros recuperados: 12 | |
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| Zhao,Yongliang; Dong,Zongming; Li,Tengfei; Huang,Gang. |
| Background: The AdEasy system is a fast-track system for generating recombinant adenoviruses using the efficient homologous recombination machinery between shuttle and adenovirus backbone plasmids in Escherichia coli BJ5183 cells. The key step is homologous recombination in BJ5183 cells, which is driven by RecA activity. However, culture time is stringently limited to reduce the damage to recombinant plasmids by RecA activity. Therefore, rapid identification of recombinant adenoviruses within the limited time-period is critical. Results: We developed a simple negative selection method to identify recombinant adenoviruses using colony PCR, which improves the efficiency of adenovirus recombination screening and packaging. Conclusions: The negative selection... |
| Tipo: Journal article |
Palavras-chave: Adenovirus; AdEasy; Colony PCR; Homologous recombination; RecA. |
| Ano: 2014 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000100008 |
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| Agungpriyono, Dewi Ratih; Nakagawa, Michio; Morozumi, Tetsuo; Yoshihara, Shinobu; Furuoka, Hidefumi; Matsui, Takane; アグリプリヨノ, デヴィ ラテイ; 中川, 迪夫; 両角, 徹雄; 吉原, 忍; 古岡, 秀文; 松井, 高峯. |
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Palavras-chave: Adenovirus; Encephalitis; Inclusion body; Nephritis; Swine. |
| Ano: 1997 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1326 |
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| BRUZZONE,MARISOL; BARRO,MARIO; SPENCER,EUGENIO. |
| Adenovirus genotype 7h was previously reported to be originated from a recombination event between adenovirus genotypes 7p and 3p. Based on those findings, further characterization of other adenovirus 7h strains become important to determine whether all adenovirus 7h strains arose from a single recombinational event. To explore such a possibility, 160 clinical isolates were studied after developing a PCR assay using a primer set designed to amplify the region corresponding to E3-7,7 Kd of adenovirus ADV 7p and E3-9 Kd of adenovirus 3p. The assay was able to differentiate most of the subgenus B strains from adeno 7h with the genotype 3d. The study of several adenovirus 7h clinical isolates revealed the existence of three variants of adeno7h. One of the... |
| Tipo: Journal article |
Palavras-chave: Adenovirus. |
| Ano: 2001 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000200010 |
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| Paulini,Inarei; Siqueira-Silva,Joselma; Thomaz,Luciana; Rocha,Leticia; Harsi,Charlotte; Bellei,Nancy; Granato,Celso. |
| Abstract Human adenoviruses comprise an important group of etiologic agents that are responsible for various diseases in adults and children, such as respiratory, ocular, gastroenteric, and urinary infections. In immunocompromised and organ-transplanted individuals, these agents can cause generalized infections. Rapid diagnostic methods for detecting these infectious agents are not widely available.The aim of this work was to produce monoclonal and polyclonal anti-adenovirus antibodies to be used in a rapid diagnostic test for respiratory infections.Adenovirus hexons were satisfactorily purified by ultracentrifugation and chromatography. After virus purification, anti-hexon monoclonal antibodies were produced and characterized, following classical methods.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Adenovirus; Monoclonal antibodies; Immunochromatographic test. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000500500 |
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| Akçay,Emine; Çarhan,Ahmet; Hondur,Gözde; Tufan,Zeliha Koçak; Duru,Necati; Kılıç,Selçuk; Ensari,Ezgi Naz; Uğurlu,Nagihan; Çağıl,Nurullah. |
| Abstract Background: Viral conjunctivitis are the most frequent infections in ophthalmology clinics. The diagnosis is usually relying on clinical findings and medical history. However, topical antibiotics are often used unnecessarily addition to symptomatic treatment because of unsure agents. We aimed to detect the Adenovirus, Coxsackievirus and Enterovirus from conjunctiva and pharyngeal samples of patients. Methods: The conjunctiva and pharyngeal samples of the patients with conjunctivitis were taken by Virocult transport media and kept at -80 ºC up to study day. Adenovirus spp, Enterovirus 70 and Enterovirus 71, Coxsackie A24 and Coxsackie A16 were detected by real-time PCR. Samples from healthy health care workers of ophthalmology clinic were used... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Adenovirus; Conjunctivitis; Viral; PCR. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000400391 |
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| Da-Costa,R.C.; Vieira,I.L.; Hunger,A.; Tamura,R.E.; Strauss,B.E.. |
| The immune stimulatory and anti-neoplastic functions of type I interferon have long been applied for the treatment of melanoma. However, the systemic application of high levels of this recombinant protein is often met with toxicity. An approach that provides localized, yet transient, production of type I interferon may overcome this limitation. We propose that the use of mesenchymal stem cells (MSCs) as delivery vehicles for the production of interferon-β (IFNβ) may be beneficial when applied together with our cancer gene therapy approach. In our previous studies, we have shown that adenovirus-mediated gene therapy with IFNβ was especially effective in combination with p19Arf gene transfer, resulting in immunogenic cell death. Here we showed that MSCs... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Gene therapy; Immunotherapy; Stem cell therapy; Adenovirus; P53; Interferon-β. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000300601 |
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| Melgaço,Fabiana Gil; Corrêa,Adriana Abreu; Ganime,Ana Carolina; Brandão,Marcelo Luiz Lima; Medeiros,Valéria de Mello; Rosas,Carla de Oliveira; Lopes,Silvia Maria dos Reis; Miagostovich,Marize Pereira. |
| Abstract This study aimed to evaluate the elution-concentration methodology based on skimmed milk flocculation from three varieties of tomatoes (Solanum lycopersicum L. [globe], Solanum lycopersicum var. cerasiforme [cherry] and hybrid cocktail [grape tomato]) for further monitoring of field samples. Spiking experiments were performed to determine the success rate and efficiency recovery of human norovirus (NoV) genogroup II, norovirus murine-1 (MNV-1) used as sample process control virus and human adenovirus (HAdV). Mean values of 18.8%, 2.8% and 44.0% were observed for NoV GII, MNV-1 and HAdV, respectively with differences according to the types of tomatoes, with lower efficiency for cherry tomatoes. Analysis of 90 samples, obtained at commercial... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Adenovirus; Flocculation; Murine norovirus-1; Norovirus; Tomato. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000500034 |
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| Luz,Roger B.; Staggemeier,Rodrigo; Fabres,Rafael B.; Soliman,Mayra C.; Souza,Fernanda G.; Gonçalves,Raoni; Fausto,Ivone V.; Rigotto,Caroline; Heinzelmann,Larissa S.; Henzel,Andréia; Fleck,Juliane D.; Spilki,Fernando R.. |
| Adenoviruses are among the most promising viral markers of fecal contamination. They are frequently found in the water, sediment and soil of regions impacted by human activity. Studies of the bioaccumulation of enteric viruses in shrimp are scarce. The cities located in the northern coast of the lake systems in Southern Brazil have high urbanization and intensive farming rates, and poor sewage collection and treatment. One hundred (n = 100) Farfantepenaeus paulensis pink-shrimp specimens and 48 water samples were collected from coastal lagoons between June 2012 and May 2013. Water samples were concentrated and the shrimp, mashed. After DNA extraction, samples were analyzed by real time polymerase chain reaction (qPCR) in order to detect and quantify viral... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Adenovirus; Farfantepenaeus paulensis; Pink-shrimp; Water quality; Northern Coast of Rio Grande do Sul. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300715 |
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| Registros recuperados: 12 | |
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