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	Provedor de dados BABT (4) Thai Agricultural (3) Repositório Alice (1) BJB (1) Scientia Agricola (1) Autor Kaltchuk-Santos,Eliane (3) Bodanese-Zanettini,Maria Helena (2) Moraes,Ana Paula de (2) Pannipa Yajai (2) ARAÚJO, L. G. de (1) Augustin,Lizete (1) Baggio,Maria Irene (1) Bered,Fernanda (1) Bonadese-Zanettini,Maria Helena (1) Brammer,Sandra Patussi (1) Callegari- Jacques,Sidia Maria (1) Callegari-Jacques,Sídia Maria (1) Cardoso,Milena Barcelos (1) Carvalho,Fernando Irajá Felix de (1) Cesaro,Taniela De (1) FERREIRA,A. G. (1) Hu,Ching -yeh (1) Iorczeski,Edson Jair (1) Jirawat Sanitchon (1) Kaltchuk- Santos,Eliane (1) Mais... Palavra-chave Anther culture (10) Androgenesis (3) Doubled haploids (2) Glycine max (2) Microsporogenesis (2) Rice improvement (2) Soybean (2) การเพาะเลี้ยงอับละอองเรณู (2) สายพันธุ์แท้ (2) Antera (1) Arroz (1) Binucleate pollen grains (1) Biotechnology (1) Blast resistance (1) Brusone (1) Callus formation (1) Culture media (1) Doença de planta (1) Duplo haplóide (1) Haploid breeding (1) Mais... Tipo do documento Info:eu-repo/semantics/article (5) Collection (3) Artigo de periódico (1) Info:eu-repo/semantics/report (1) Ano 2015 (1) 2011 (2) 2010 (1) 2009 (1) 2008 (1) 2007 (1) 2004 (1) 2003 (1) 2000 (1) Mais... País Brazil (7) Thailand (3) Idioma Inglês (7) Thailandês (3)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 10 Primeira ... 1 ... Última Haplodiploid androgenetic breeding in oat: genotypic variation in anther size and microspore development stage Scientia Agricola Cesaro,Taniela De; Baggio,Maria Irene; Zanetti,Silvia Andréia; Suzin,Marilei; Augustin,Lizete; Brammer,Sandra Patussi; Iorczeski,Edson Jair; Milach,Sandra Cristina Kothe. Oat (Avena spp.) is poorly responsive to the haplodiploidization process, which leads to the production of homozygous lines in one step, increasing breeding efficiency. Androgenetic haploids in small grain cereal crops are obtained from microspores cultured at the mononucleate stage, which can be identified by the size of anthers. In order to identify the appropriate anther size for in vitro culture, microspore cytological analyses were made in Avena sativa cultivars UPF 7, UPF 18, UFRGS 14, Stout and Avena sterilis CAV 3361, cultivated in growth chamber under controlled light and temperature conditions. Variation was observed within and among genotypes for anther size at each microspore developmental stage and according to the position of spikelets in the... Tipo: Info:eu-repo/semantics/report Palavras-chave: Androgenesis; Anther culture; Biotechnology; Haploid breeding; Microspores. Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162009000100016 อิทธิพลของอาหารที่มีต่อการเพาะเลี้ยงอับละอองเกสรตัวผู้ในถั่วลิสง Thai Agricultural Jirawat Sanitchon; Viboon Pensuk. Production of haploids is an important tool for the rapid generation of homozygous breeding lines. Experiments have been conducted with the aim of developing a method for haploids peanut plant production by us -ing anther culture. Peanut genotype Tainan 9 was tested with different media compositions. The suitable medium for anther culture of peanut in this study was N6 The callus was white to creamish at the time of induction with loose to compact form. Callus proliferation was well developed in MS medium supplemented with 4 mg/l IAA and 2 mg/l Kinetin. In some cases, green spots were observed in the callus. Tipo: Collection Palavras-chave: Peanut; Anther culture; Haploids; ถั่วลิสง; พันธุ์ไทนาน 9; เฮพลอยด์; การเพาะเลี้ยงอับละอองเกสรตัวผู้; สายพันธุ์แท้; แคลลัส. Ano: 2011 URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5165 การเพาะเลี้ยงอับละอองเรณูข้าวเพื่อสร้างสายพันธุ์แท้ให้ต้านทานโรคไหม้ Thai Agricultural Pannipa Yajai; Kulchana Ketsuwan. Anther culture (AC) technology is being used to conduct rice blast resistant true breeding line at Phrae Rice Research Center in 2012. Five crosses from irrigated rice research and development program were cultured on N6 formula media to induce callus formation in the dark room, 20-250C and light intensity about 2000 Lux for 1-2 months and followed by MS media for 1-2 months. Then, the plantlet was transplanted to nursery and 96 progenies of five crosses were planted to observational trial in the field during wet season, 2014. The evaluation of blast reaction was conducted at Phrae Rice Research Center using mixed isolates of blast pathogen from Phrae province. The results show that one line (PRE12111-AC-1-5) is highly resistance (HR) and 26 lines are... Tipo: Collection Palavras-chave: Rice improvement; Anther culture; Rice blast; Blast resistance; การปรับปรุงพันธุ์ข้าว; การเพาะเลี้ยงอับละอองเรณู; โรคไหม้; ความต้านทานโรคไหม้. Ano: 2015 URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5896 การเพาะเลี้ยงอับละอองเรณูข้าวเพื่อสร้างสายพันธุ์แท้ให้ต้านทานแมลงบั่ว Thai Agricultural Pannipa Yajai; Noppadol Somna. To improved rice gall midge (GM) resistance by F1 progenies’ anther culture, conducted at Phrae Rice Research Center (PRE_RRC) in 2009 dry and wet season. Sixteen crosses from irrigated rice R&D program and three crosses from rainfed rice R&D program were culture in N6 formula media to induced callus formation in dark room, 20-250C temperature and 2000 Lux light intensity for 1-2 month duration. Followed by MS media culture (Murashige and Skoog, 1962) in 2000 Lux light intensity for 1-2 month duration. Then, the plantlets were transplanted to nursery trial and 53 progenies of 5 IR crosses, and 8 progenies of 3 RR crosses were gathered and planted to observational trial in the field during 2010 wet season, together with GM reaction nursery at... Tipo: Collection Palavras-chave: Anther culture; Rice gall midge; Rice improvement; Resistance Line; ข้าว; แมลงบั่ว; การเพาะเลี้ยงอับละอองเรณู; พันธุ์ต้านทาน; การพัฒนาพันธุ์ข้าว; การปรับปรุงพันธุ์; การเจริญเติบโตเป็นต้นอ่อน; ความต้านทานต่อแมลงบั่ว; สายพันธุ์แท้. Ano: 2011 URL: http://anchan.lib.ku.ac.th/agnet/handle/123456789/3082 Marker-assisted selection for the rice blast resistance gene Pi-ar in a backcross population. Repositório Alice ARAÚJO, L. G. de; PRABHU, A. S.; PEREIRA, P. A. A.; SILVA, G. B. da. A doubled-haploid (DH) population, obtained by anther culture of F 1 plants from a cross between a highly susceptible rice cultivar Lijiangxintuanheigu and the resistant somaclone (SC09), of the cultivar Araguaia, was used to identify RAPD markers linked to the blast resistance gene Pi-ar. The 86 DH plants, inoculated with the race IB-9 of Magnaporthe oryzae, segregated in 1:1 ratio of resistant and susceptible plants. Of the 67 primers used 31 produced DNA profiles that differentiated resistant and susceptible bulks as well as the parental cultivars. The resistance gene was found linked to the primer OPS162072 (‘AGGGGGTTCC’) at a distance of 3.6 cM. The selection efficiency of this primer was assessed in a BC3 F1 population derived from another cross... Tipo: Artigo de periódico Palavras-chave: Duplo haplóide; Arroz; Oryza sativa; Antera; Brusone; Doença de planta; Marcador molecular; Rice; Doubled haploids; Anther culture; Magnaporthe grisea. Ano: 2010 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/862208 Association between floral bud size and developmental stage in soybean microspores BABT Lauxen,Mozart da Silva; Kaltchuk- Santos,Eliane; Hu,Ching -yeh; Callegari- Jacques,Sidia Maria; Bodanese-Zanettini,Maria Helena. This study was carried out to establish the association between floral bud size and the corresponding microspore developmental stages for Brazilian soybean cultivars. Microspore developmental stage distributions were examined in young buds from cv Década, IAS5 and RS7. The data indicated that for a given bud-size group, the microspores of different cultivars were at different developmental stages, with cv RS7 and Década distributed at the youngest and cv IAS5 at the most advanced stages. Microspore stages distribution were also compared among the ten anthers of the same bud of the above cultivars. The ten anthers from a given bud were clearly distributed at different developmental stages. Caution should be exercised when adopting the standard anther... Tipo: Info:eu-repo/semantics/article Palavras-chave: Glycine max; Soybean; Microsporogenesis; Anther culture. Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000400004 Effect of temperature shock on soybean microspore embryogenesis BABT Moraes,Ana Paula de; Bonadese-Zanettini,Maria Helena; Callegari-Jacques,Sídia Maria; Kaltchuk-Santos,Eliane. In this study the effect of cold and heat shock on androgenetic induction in soybean anther culture was tested. Anthers of soybean were subjected to 4 and 33ºC, while the anthers used for control were maintained at 25ºC. Cytological analysis done during the 30 days of culture showed that frequencies of symmetrical binucleated and multicellular pollen grain did not differ among temperature treatments. Multicellular pollen grain could be formed by symmetrical division, as well as by assymetrical division. In relation to the embryo induction, the results of the treatments did not differ too. These results suggested that these treatments did not induce a sporophytic pathway in soybean. Tipo: Info:eu-repo/semantics/article Palavras-chave: Anther culture; Soybean; Androgenesis; Symmetrical mitosis; Temperature shock; Stress. Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000400006 Morphological markers for microspore developmental stage in maize BABT Moraes,Ana Paula de; Bered,Fernanda; Carvalho,Fernando Irajá Felix de; Kaltchuk-Santos,Eliane. The use of maize in anther culture has been limited because only few genotypes presented a high androgenetic potential. Obtaining the proper stage of microspore development at culture initiation is one of the most crucial factors for success in the androgenesis. For Brazilian maize genotypes there are no studies reporting a correlation between cytological features and morphological parameters. In this study, morphological parameters were recorded and associated with cytological specific stages of the the microsporogenesis in two Brazilian maize genotypes that were sowed in different places (field and growing chamber). For both genotypes, the plants of the growing chamber presented a delay in development. Spikelets length and anther length/spikelet length... Tipo: Info:eu-repo/semantics/article Palavras-chave: Androgenesis; Anther culture; Microsporogenesis; Zea mays. Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000500006 Evaluation of gelling agents on anther culture: response of two soybean cultivars BABT Cardoso,Milena Barcelos; Bodanese-Zanettini,Maria Helena; Mundstock,Elsa Cristina de; Kaltchuk-Santos,Eliane. Anthers of two soybean cultivars were cultured in B5 long basal culture media gelled with agarose or PhytagelTM. Cytological examinations of the anthers were carried out during the first 45 days of culture to assay the viability and developmental stage of microspores. Frequency of callus formation was recorded at 45 days of culture. The analysis of variance of the microspore viability assay showed significant Cultivar X Gelling Agent X Day of Culture interactions. The frequencies of viable microspores decreased significantly with time of culture, within each cultivar and gelling agent tested. The interaction Day X Cultivar was significant for the frequencies of binucleate symmetrical grains and multinucleate/multicellular structures. The effect of gelling... Tipo: Info:eu-repo/semantics/article Palavras-chave: Anther culture; Glycine max; Culture media; Binucleate pollen grains; Multinucleate/multicellular structures; Callus formation. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000700004 Ontogenetic events in androgenesis of Brazilian barley genotypes BJB SILVA,A. L. S. da; MORAES-FERNANDES,M. I.; FERREIRA,A. G.. This paper describes a simple procedure for obtention of barley (Hordeum vulgare L.) doubled haploid plants from Brazilian hybrid genotypes. Anatomical and histological examinations showed the reversion of barley pollen to an sporophytic mode of development. A sequence of mitosis led to the formation of multicellular pollen grains. Regeneration of plants occurred either by direct embryogenesis or callus formation followed by differentiation through direct embryogenesis or organogenesis. Plants were formed in the same medium used for induction dispensing an additional regeneration step. This procedure makes doubled haploid production simpler and faster. Plantlets were transferred to another medium for rooting and after that planted in pots with vermiculite... Tipo: Info:eu-repo/semantics/article Palavras-chave: Anther culture; Doubled haploids. Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0034-71082000000200016 Registros recuperados: 10 Primeira ... 1 ... Última

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