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Menegat, Francieli Bortoluzzi; Baldo, Cristiani; Celligoi, Maria Antonia Pedrine Colabone; Buzato, João Batista. |
Asparaginase is an enzyme used in clinical treatments as a chemotherapeutic agent and in food technology to prevent acrylamide formation in fried and baked foods. Asparaginase is industrially produced by microorganisms, mainly gram-negative bacteria. Zymomonas mobilis is a Gram-negative bacterium that utilizes glucose, fructose and sucrose as carbon source and has been known for its efficiency in producing ethanol, sorbitol, levan, gluconic acid and has recently aroused interest for asparaginase production. Current assay optimizes the production of Z. mobilis asparaginase by continuous fermentation using response surface experimental design and methodology. The studied variables comprised sucrose, yeast extract and asparagine. Optimized condition obtained... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Sucrose; Asparagine; Yeast extract; Enzyme; Microbial production; Factorial design. |
Ano: 2016 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/31057 |
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Queiroz,Helena Müller; Sodek,Ladaslav; Haddad,Claudia Regina Baptista. |
Soybean plants cultivated with 50, 100 and 200 mM of NaCl, revealed that root growth was less affected by salinity than shoots. Salinity led to a reduction in leaf area and an increase in water content of the roots. These factors could contribute to the adaptation of the plant, improving its hydration. Although nitrate and free amino acid levels were reduced by salt treatment in roots, protein content of leaves was not altered. Salinity led to alterations in xylem amino acid composition, with increases in Ser, Ala, Gaba and Pro and a decrease in Asn. Similar changes were seen for Asn and Ser in roots together with a much stronger increase in Gaba. It is suggested that the decline in Asn reflects its conversion to Ala and Gaba (via Glu) in the roots while... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Soybean; Stress; Amino acid transport; Asparagine; Alanine; Γ-aminobutyrate. |
Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000600002 |
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