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| Silva,Claudênia Ferreira da; Uesugi,Carlos Hidemi; Blum,Luiz Eduardo Bassay; Marques,Abi Soares dos Anjos; Ferreira,Marisa Álvares da Silva Velloso. |
| ABSTRACT: Erwinia psidii causes bacterial blight of guava ( Psidium guajava ), an important disease of this crop in Brazil. The pathogen affects branches and twigs of guava trees, reducing yield significantly. Bacterial dissemination often occurs through contaminated but asymptomatic propagating plant material. The objectives of this research were to evaluate the use of BIO-PCR and conventional PCR to detect E. psidii in inoculated guava plants grown in a greenhouse and in symptomatic and asymptomatic trees from guava orchards. Erwinia psidii strain IBSBF 1576 was inoculated (107CFU mL-1) into young guava shoots and plant tissue was analysed at 0, 5, 10, and 15 days after inoculation. Symptoms were observed after 5 days and all inoculated shoots were PCR... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: BIO-PCR; Diagnosis; Guava bacterial blight; PCR; Psidium guajava. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000901528 |
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