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| Registros recuperados: 11 | |
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| López Garrido, Serafín Jacobo. |
| El objetivo del presente estudio fue obtener un cultivo puro de la bacteria ruminal Streptococcus bovis a partir de líquido ruminal de ovinos alimentados con una dieta alta en sorgo (70 % en base seca). Además, se analizó su actividad fermentativa (producción de AGV y lactato) y el proceso de liofilizado como método de conservación, y se realizaron ensayos para expresar su bacteriófago. Para el aislamiento se usó un medio comercial para Streptococcus spp. (Merck) preparado bajo anaerobiosis. Para la identificación se usó el sistema API 20 Strep, reacción catalasa, morfología celular y tinción de Gram. Con la prueba API 20 Strep se comprobó que la bacteria aislada pertenece a la especie S. bovis. Esta bacteria tiene forma esférica a ovoide, de 0.8... |
| Tipo: Tesis |
Palavras-chave: Rumen; Streptococcus bovis; Aislamiento; Iiofilización; Bacteriófago; Doctorado; Ganadería; Isolation; Lyophilization; Bacteriophage. |
| Ano: 2008 |
URL: http://hdl.handle.net/10521/1402 |
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| Parra,Bárbara; Robeson,James. |
| Background: Ideally, bacteriophages of pathogenic bacterial hosts should be polyvalent to be able to replicate in an alternative nonpathogenic bacterium. Thus, accidental infection by the original host can be avoided when bacteriophage lysates are used in biocontrol protocols. Results: From 15 wastewater samples, collected at different sites in the V Region in Chile, we selected three bacteriophages (FC, FP, and FQ) capable of productively infecting Salmonella enterica serovar Choleraesuis. By transmission electron microscopy (TEM) observation, the bacteriophages were found to belong to the order Caudoviridae. Molecular analyses indicated that FC, FP, and FQ contained double-stranded DNA genomes, of sizes similar to bacteriophage P22, and distinct... |
| Tipo: Journal article |
Palavras-chave: Bacteriophage; Polyvalent; Salmonella enterica serovar Choleraesuis. |
| Ano: 2016 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000300011 |
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| Derolez, Valerie; Serais, Ophelie; Caprais, Marie-paule; Le Saux, Jean-claude; Messiaen, Gregory. |
| Microbes regularly contaminate Thau lagoon shellfish. Between 1997 and 2007, 18% of the analyses carried out in dry weather recorded more than 230 Escherichia coli / 100 g of bivalves (Ifremer French microbial monitoring network), leading the administration to restrict the shellfish harvest area, including suspension of production. This study aims to determine, the contribution of seabirds, which roost at night on the Thau lagoon shellfish growing frames, to shellfish faecal pollution in dry weather.Data were collected in 2008 from both the drainage area (pollution input) and the lagoon (water quality and microbial monitoring, seabird counts) in dry weather. The data showed that, with no watershed input or lagoon resurgence, microbial contaminants were... |
| Tipo: Text |
Palavras-chave: Escherichia coli; Origine aviaire; Coquillage; Contamination fécale; Étang de Thau; Oiseau de mer; Bactériophage; Dépistage de la source microbienne; Temps sec; Escherichia coli; Avian source; Shellfish; Faecal contamination; Thau lagoon; Seabird; Bacteriophage; Microbial source tracking; Dry weather. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/00025/13593/10659.pdf |
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| Harwood, Valerie J.; Boehm, Alexandria B.; Sassoubre, Lauren M.; Vijayavel, Kannappan; Stewart, Jill R.; Fong, Theng-theng; Caprais, Marie-paule; Converse, Reagan R.; Diston, David; Ebdon, James; Fuhrman, Jed A.; Gourmelon, Michele; Gentry-shields, Jennifer; Griffith, John F.; Kashian, Donna R.; Noble, Rachel T.; Taylor, Huw; Wicki, Melanie. |
| An inter-laboratory study of the accuracy of microbial source tracking (MST) methods was conducted using challenge fecal and sewage samples that were spiked into artificial freshwater and provided as unknowns (blind test samples) to the laboratories. The results of the Source Identification Protocol Project (SIPP) are presented in a series of papers that cover 41 MST methods. This contribution details the results of the virus and bacteriophage methods targeting human fecal or sewage contamination. Human viruses used as source identifiers included adenoviruses (HAdV), enteroviruses (EV), norovirus Groups I and II (NoVI and NoVII), and polyomaviruses (HPyVs). Bacteriophages were also employed, including somatic coliphages and F-specific RNA bacteriophages... |
| Tipo: Text |
Palavras-chave: Virus; Bacteriophage; Water quality; Fecal pollution; Validation. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00171/28197/27786.pdf |
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| Yeboah, Osei-Agyeman; Goktepe, Ipek; Naanwaab, Cephas B.; Kyei, Foster Ofori. |
| The United States is remarkably safe, when it comes to food supply. Nevertheless, food can become contaminated with a variety of germs. According to reports by a food safety group of the Centers for Disease Control and Prevention, food-borne illness caused by bacteria such as E. coli and salmonella not only take a huge toll on American consumers’ health but they cost the United States an estimated $152 billion annually in health care and other losses. To curb this increasing phenomenon, there has been a reintroduction of bacteriophage in the treatment of bacteria on raw foods. This study utilized a survey questionnaire administered by telephone to consumers in four different states; Alabama, Georgia, North Carolina, and South Carolina. In this study, as... |
| Tipo: Presentation |
Palavras-chave: Willingness-to-pay; Bacteriophage; Fresh produce; Logit model; Agribusiness; Agricultural and Food Policy; Consumer/Household Economics; Environmental Economics and Policy. |
| Ano: 2012 |
URL: http://purl.umn.edu/119733 |
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| Lee,HY; Biswas,D; Ahn,J. |
| ABSTRACT This study was designed to assess the role of bacteriophage P22 in the adhesion, invasion, intracellular survival of, and cellular immune response to Salmonella Typhimurium in intestinal epithelial INT-407 and chicken macrophage-like HD11 cells. The ability of S. Typhimurium to adhere, invade, and survive to INT-407 and HD11cells was evaluated under Salmonella infection alone (control), phage treatment followed by Salmonella infection (PS), Salmonella infection followed by phage treatment (SP), and a combination treatment with Salmonella and phage (S+P). The number of S. Typhimurium associated on INT-407 cells was reduced from 4.2 to 2.7 log cfu/cm2 by phage treatment (SP). The number of intracellular S. Typhimurium within INT-407 cells was... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Adhesion; Bacteriophage; Invasion; Salmonella; Toll-like receptor. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2015000400427 |
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| Silva,Elaine Nóbrega Gibson; Figueiredo,Ana Cláudia Leite; Miranda,Fernanda Araújo; Almeida,Rogeria Comastri de Castro. |
| The purpose of this study was to determine the effect of bacteriophage P100 on strains of Listeria monocytogenes in artificially inoculated soft cheeses. A mix of L. monocytogenes 1/2a and Scott A was inoculated in Minas Frescal and Coalho cheeses (approximately 10(5) cfu/g) with the bacteriophage added thereafter (8.3 x 10(7) PFU/g). Samples were analyzed immediately, and then stored at 10 ºC for seven days. At time zero, 30 min post-infection, the bacteriophage P100 reduced L. monocytogenes counts by 2.3 log units in Minas Frescal cheese and by 2.1 log units in Coalho cheese, compared to controls without bacteriophage. However, in samples stored under refrigeration for seven days, the bacteriophage P100 was only weakly antilisterial, with the lowest... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Food safety; Listeria; Biocontrol; Bacteriophage; Cheese. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100003 |
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| Registros recuperados: 11 | |
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