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Registros recuperados: 57
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Effect of temperature changes on the cercarial-shedding rate of two trematodes Iheringia, Sér. Zool.
Achiorno,Cecilia L.; Martorelli,Sergio R..
ABSTRACT Since temperature is a key condition in the initiation of cercarial emergence, the objective of the work reported here was to evaluate the effect of that variable on the shedding of two morphologic and taxonomic types of cercariae. Bioassays were accordingly performed with snails naturally infected with either monostome or pleurolophocercous cercariae because those two cercarial types were found to be the most prevalent in the environment under study. The snails were placed in 6-ml circular plastic dishes with soft plastic stoppers containing 3 ml of reconstituted water and then sequentially exposed for 24 h to a 14:10-h light:dark cycle at 20, 25, or 30 °C. The data were analyzed at a significance level of p <0.05 by means of the InfoStat...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Parasites; Cercarial emergence; Freshwater; Temperature; Bioassay.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0073-47212016000100220
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Evaluation du potentiel nutritif des eaux de la baie de Vilaine pendant l'été 1987 ArchiMer
Le Dean, Loic; Truquet, Philippe; Lassus, Patrick.
The growth potential study of waters of the Vilaine bay was realized from march to september 1987 and for three sampling stations. The growth of depleted test cells of Skeletonema costatum was estimated and compared from existing nutrients pools for aIl water samples. As a first result it appears that inorganic nitrogen could stimulate the growth during spring while no clear relationship is observed between the algal growth and other hydrological parameters during spring or summer periods. Nevertheless these first results need further corroboration.
Tipo: Text Palavras-chave: Potentiel nutritif; Baie de Vilaine; Skeletonema costatum; Test biologique; Growth potential; Vilaine Bay; S. costatum; Bioassay.
Ano: 1988 URL: http://archimer.ifremer.fr/doc/00139/25037/23146.pdf
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Limiting nutrients of oyster pond seawater in the Marennes-Oleron region for Haslea ostrearia : applications to the mass production of the diatom in mesocosom experiments ArchiMer
Turpin, Vincent; Robert, Jean-michel; Goulletquer, Philippe.
Bioassays were carried out with the 'blue diatom' Haslea ostrearia Simonsen, which is responsible for oyster greening during the fattening period of Crassostrea gigas Thunberg in oyster ponds. Samples of seawater were taken from two oyster ponds: one without oysters and the other with 20 oysters per m_, maximal density allowed by the French AFNOR norm for 'refinement'. The aims were to clarify the nutrient requirements of this diatom, also to elucidate the eventual influence of C. gigas at this density on the seawater fertility and to envisage the mass production of this diatom by pond fertilization. Examination of cell numeric densities at the end of bioassays allows us to conclude that silicate was the first limiting nutrient, closely followed by...
Tipo: Text Palavras-chave: Marennes Oleron; Oyster ponds; Mesocosm experiment; Bioassay; Haslea ostrearia; Bacillariophyceae; Limiting nutrients; Marennes Oléron; Claires à huîtres; Expérience en mésocosmes; Bioessai; Haslea ostrearia; Bacillariophyceae; Nutriments limitants.
Ano: 1999 URL: http://archimer.ifremer.fr/doc/1999/publication-3096.pdf
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Measuring the biological impact of drilling waste on the deep seafloor: an experimental challenge ArchiMer
Lelchat, F.; Dussauze, M.; Lemaire, P; Theron, M.; Toffin, Laurent; Le Floch, Stephane.
The depletion of traditional oil fields is driving the oil & gas industry to explore new exploitation sites previously considered as unprofitable. Deep-sea oil fields represent one of these new areas of exploitation. Well drilling during exploration and production operations generate large quantities of drilling waste whose biological impact on the deep-sea floor remains largely unknown. Because of the harsh abiotic factors characterizing this environment, the evaluation of this impact remains challenging. High hydrostatic pressure is the prominent factor which will affect in-situ biological processes. This review will examine the feedback on the various strategies used to evaluate the biological impact of deep-sea drilling waste deposition as well as...
Tipo: Text Palavras-chave: Drill cutting; Drilling waste; Ecotoxicology; Bioassay; Hydrostatic pressure; Deep-sea; Environmental assessment.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00603/71561/69988.pdf
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A rapid quantitative fluorescence-based bioassay to study allelochemical interactions from Alexandrium minutum ArchiMer
Long, Marc; Tallec, Kevin; Soudant, Philippe; Lambert, Christophe; Le Grand, Fabienne; Sarthou, Geraldine; Jolley, Dianne; Hegaret, Helene.
Harmful microalgal blooms are a threat to aquatic organisms, ecosystems and human health. Toxic dinoflagellates of the genus Alexandrium are known to produce paralytic shellfish toxins and to release bioactive extracellular compounds (BECs) with potent cytotoxic, hemolytic, ichtyotoxic and allelopathic activity. Negative allelochemical interactions refer to the chemicals that are released by the genus Alexandrium and that induce adverse effects on the physiology of co-occurring protists and predators. Releasing BECs gives the donor a competitive advantage that may help to form dense toxic blooms of phytoplankton. However BECs released by Alexandrium minutum are uncharacterized and it is impossible to quantify them using classical chemical methods....
Tipo: Text Palavras-chave: Harmful algal bloom; Alexandrium; Allelopathy; Cytotoxicity; PAM fluorometry; Bioassay.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00453/56424/58116.pdf
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Effects of dissolved mercury on embryogenesis, survival, growth and metamorphosis of Crassostrea gigas oyster larvae ArchiMer
Beiras, Ricardo; His, Edouard.
The effects of mercury (Hg) concentrations ranging from 0 (control) to 1024 µg 1-1 upon embryogenesis, survival, growth and metamorphosis of Crassostrea gigas (Thunberg) oyster larvae were investigated. Embryogenesis was abnormal in 50 % of the individuals at 11 µg 1-1 . The 48 h LD50 for D·shaped, umbonate and pediveliger larvae were 33, 115 and 200 µg 1-1 respectively. The increase in LD50 was partially explained by the larval weight increase, although weight-specific tolerance to Hg was higher in smaller larvae. Growth, the most sensitive physiological process studied, was significantly retarded at 4 µg 1-1. The metamorphosis rate was significantly reduced when competent pediveligers were exposed to 64 µg 1-1 for 48 h prior to the addition of the...
Tipo: Text Palavras-chave: Mercury; Oyster; Larvae; Embryo; Bioassay; Ecotoxicology; Crassostrea gigas.
Ano: 1994 URL: http://archimer.ifremer.fr/doc/00037/14867/12191.pdf
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Mytilus galloprovincialis as bioindicator in embryotoxicity testing to evaluate sediment quality in the lagoon of Venice (Italy) ArchiMer
Annamaria, V; Chiara, L; Alessandra, A; Alvise, B; His, Edouard; Francesco, G.
The possibility of using Mytilus galloprovincialis from natural populations of the lagoon of Venice in toxicity bioassays based on embryo development, according to international standard methods, was investigated. In order to valuate this method for the lagoon, iterative steps including evaluation of reproducibility, sensitivity and discriminatory capacity towards some pure substances and environmental samples were performed. Evaluation of sensitivity towards a reference toxicant (copper) evidenced good replicability and repeatability. Results showed that natural population is better than hatchery population for performing bioassays. Evaluation of applicability to elutriates revealed that the method is able to discriminate among sediments of the lagoon of...
Tipo: Text Palavras-chave: Elutriates; Autochthonous population; Reproducibility; Bioassay.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-4200.pdf
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A method to assess algicidal activity of microalgal extracts coupling microalgae produced in stirred closed photobioreactor operating in continuous with pulse amplitude modulated (PAM) fluorometry ArchiMer
Menguy, Eva; Dumontet, Vincent; Coulombier, Noémie; Meriot, Vincent; Le Dean, Loic; Barthelemy, Vanille; Jauffrais, Thierry.
We describe in the present study a quick and reliable method based on chlorophyll a fluorescence to assess putative algicidal effect of different microalgal extracts. We couple microalgal production under chemostat cultivation mode to continuously produce a given microalgae species (e.g. Dunaliella salina in this study) at a stable physiological state to ease comparison between extracts tested; with a non-destructive method based on chlorophyll a fluorescence. Pulse amplitude modulated (PAM) fluorometry was used to assess over time the effect of different microalgal crude extracts on the efficiency of the photosystem II (PSII) of a tested microalgae (Dunaliella salina). • Microalgal production at stationary phase in stirred closed photobioreactor (PBR)...
Tipo: Text Palavras-chave: Bioassay; Cytoxicity; Microalgae; Quantum efficiency; Fv/Fm; Non photochemical quenching; NPQ; Electron transport rate; Phytochemistry; Anti algal.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00643/75524/76400.pdf
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Effects of dissolved mercury on embryogenesis, survival and growth of Mytilus galloprovincialis mussel larvae ArchiMer
Beiras, R; His, Edouard.
The effects of Hg concentrations ranging from 0 (control) to 1024 mu g l(-1) upon embryogenesis, survival and growth of Mytilus galloprovincialis mussel larvae were investigated. Embryogenesis was abnormal in 50% of the individuals at 10 mu g l(-1). The 48 h LC(50) for D-shaped, early umbonate, late umbonate and eyed larvae were 51, 164, 322 and 383 pg l(-1) respectively. The LC(50) was an allometric function of ash-free dry weight with exponent b = 0.60. Larval growth was significantly reduced after 6 d exposure to 4 mu g Hg l(-1) and after 10 d exposure to 2 mu g l(-1). No significant differences in lethal or sublethal sensitivity to Hg were found between M. galloprovincialis and previously studied Crassostrea gigas embryos and larvae. The implications...
Tipo: Text Palavras-chave: Mercury; Mytilus; Mussel; Larva; Growth; Embryo; Bioassay; Ecotoxicology.
Ano: 1995 URL: http://archimer.ifremer.fr/doc/00258/36946/35665.pdf
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Exploring the chemodiversity of tropical microalgae for the discovery of natural antifouling compounds ArchiMer
Réveillon, Damien; Tunin-ley, Alina; Grondin, Isabelle; Othmani, Ahlem; Zubia, Mayalen; Bunet, Robert; Turquet, Jean; Culioli, Gérald; Briand, Jean-françois.
Marine microalgae and cyanobacteria have largely been studied for their biotechnological potential and proved their ability to produce a wide array of bioactive molecules. We investigated the antifouling potential of unexplored benthic tropical microalgae using anti-adhesion and toxicity bioassays against two major micro- and ma crobiofoulers, namely bacteria and barnacles. Fifty strains belonging to six phyla [Cyanobacteria, Miozoa (Dinoflagellata), Bacillariophyta, Cryptophyta, Rhodophyta and Haptophyta] were isolated from southwestern Islands of the Indian Ocean. They were chosen in order to represent as much as possible the huge biodiversity of such a rich tropical ecosystem. The associated chemodiversity was highlighted by both NMR- and LC-MS-based...
Tipo: Text Palavras-chave: Microalgae; Antifouling; Bioassay; Chemodiversity; Bioprospecting; Metabolomics.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00454/56608/58333.pdf
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Eléments nutritifs limitant la biomasse phytoplanctonique d'une lagune tropicale de Côte d'Ivoire. Comparaison des méthodes d'estimation ArchiMer
Dufour, P.; Cremoux, Jl.; Slepoukha, M..
"In vitro" enrichment experiments on natural populations have been used to determine the nutrients controlling the potential biomass of phytoplancton in the Ebrié coastal lagoon. The limiting nutrients were generally nitrogen, phosphorus and carbon, in this order ; in three cases out of eleven, P and N act simultaneously ; in an extreme eutrophication case, N, P and C were all limiting. No effect was observed following addition of metals, vitamins or chelators. The limitations deduced from the enrichment experiments show a. good agreement with the elementary composition of the external medium ; in waters potentially limited by N alone, the dissolved inorganic nutrients have a N : P ratio lower than 25. The agreement is poorer when the sestonic N : P ratio...
Tipo: Text Palavras-chave: Lagune; Estuaire; Zone tropicale; Phytoplancton; Biomasse; Eléments limitants; Tests biologiques; Composition chimique; Coastal lagoon; Estuary tropical zone; Phytoplankton; Biomass; Limiting nutrients; Bioassay; Chemlcal composition.
Ano: 1979 URL: http://archimer.ifremer.fr/doc/00306/41689/40898.pdf
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Integrated indicator framework and methodology for monitoring and assessment of hazardous substances and their effects in the marine environment ArchiMer
Vethaak, A. Dick; Davies, Ian M.; Thain, John E.; Gubbins, Matthew J.; Martinez-gomez, Concepcion; Robinson, Craig D.; Moffat, Colin F.; Burgeot, Thierry; Maes, Thomas; Wosniok, Werner; Giltrap, Michelle; Lang, Thomas; Hylland, Ketil.
Many maritime countries in Europe have implemented marine environmental monitoring programmes which include the measurement of chemical contaminants and related biological effects. How best to integrate data obtained in these two types of monitoring into meaningful assessments has been the subject of recent efforts by the International Council for Exploration of the Sea (ICES) Expert Groups. Work within these groups has concentrated on defining a core set of chemical and biological endpoints that can be used across maritime areas, defining confounding factors, supporting parameters and protocols for measurement. The framework comprised markers for concentrations of, exposure to and effects from, contaminants. Most importantly, assessment criteria for...
Tipo: Text Palavras-chave: Chemical measurements; Biomarker; Bioassay; Pollution effects; Biomonitoring; Environmental; Impact; MSFD; ICES; OSPAR.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00283/39429/37869.pdf
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Effects of dissolved mercury on embryogenesis, survival, growth of Mytilus galloprovincialis mussel larvae ArchiMer
Beiras, Ricardo; His, Edouard.
The effects of Hg concentrations ranging from 0 (control) to 1024 µg 1-1 upon embryogenesis, survival and growth of Mytilus galloprovincialis mussel larvae were investigated . Embryogenesis was abnormal in 50 % of the individuals at 10µg 1-1. The 48 h LC50 for D-shaped, early umbonate, late umbonate and eyed larvae were 51, 164, 322 and 383 µg 1- 1 respectively. The LC50 was an allometric function of ash-free dry weight with exponent b = 0.60. Larval growth was significantly reduced alter 6 d exposure to 4 µg Hg 1-1 and alter 10 d exposure to 2 µg 1-1. No significant differences in lethal or sublethal sensitivity to Hg were found between M. galloprovincialis and previously studied Crassostrea gigas embryos and larvae. The implications of these findings for...
Tipo: Text Palavras-chave: Mercury; Mytilus galloprovincialis; Mussel; Larvae; Growth; Embryonic development; Bioassay; Ecotoxicology.
Ano: 1995 URL: http://archimer.ifremer.fr/doc/00037/14868/12192.pdf
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Efficient, fast and inexpensive bioassay to monitor benthic microalgae toxicity: Application to Ostreopsis species ArchiMer
Pavaux, Anne-sophie; Ternon, Eva; Dufour, Louison; Marro, Sophie; Gémin, Marin-pierre; Thomas, Olivier P.; Lemée, Rodolphe.
Even though HPLC-MS is commonly used to quantify the toxin content of Ostreopsis spp. cells, there is a need to develop easy-to-use toxicological tests to set thresholds during Ostreopsis spp. blooms. The crustacean Artemia has been widely used to evaluate the presence and toxicity of chemicals and biological contaminants and we anticipated that it could also be useful to test Ostreopsis spp. toxicity. Its relevance was first assessed by investigating the variability of the toxic effects among Ostreopsis spp. strains and throughout the dinoflagellate life cycle in combination with chemical analyses of the toxinic content by UHPLC-HRMS. After testing the toxicity of fractions prepared from Ostreopsis spp. cells, the known ova- and paly-toxins were not the...
Tipo: Text Palavras-chave: Ostreopsis spp.; Anemia franciscana; Bioassay; Ecotoxicity; HPLC-HRMS.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00624/73625/73051.pdf
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Biomedical and pharmacological potential of tetrodotoxin-producing bacteria isolated from marine pufferfish Arothron hispidus (Muller, 1841) J. Venom. Anim. Toxins incl. Trop. Dis.
Bragadeeswaran,S; Therasa,D; Prabhu,K; Kathiresan,K.
Specimens of the pufferfish Arothron hispidus collected at Parangipettai, on the southeast coast of India, were subjected to bacterial isolation and identification. Three species were identified, namely Bacillus sp., Kytococcus sedentarius and Cellulomonas fimi. Partially-purified microbial filtrates exhibited hemolytic activity on chicken and human erythrocytes of O, B and AB blood groups, with maximum activity of 32 HU. The microbial filtrates also presented ATPase, Mg2+-ATPase, Na+K+-ATPase and AchE enzymatic activities of positive neuromodulation in Kytococcus sedentarius with 1300, 300.1, 1549.98 and 140.55%, in Cellulomonas fimi with 620, 300, 10 and 128.42%, and in Bacillus species with 40, 200, 849.98 and 158.69%, respectively. Toxicity symptoms...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Arothron hispidus; Tetrodotoxin; Bioassay; ATPase and AchE enzymatic activities; Anticancer activity.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000300008
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Detection of Toxoplasma gondii in the milk of experimentally infected Wistar female rats J. Venom. Anim. Toxins incl. Trop. Dis.
Costa,VM; Langoni,H.
The milk of experimentally infected rats was investigated for the presence and possible transmission of Toxoplasma gondii. Wistar (Rattus norvegicus) female rats were divided into three groups and orally inoculated with bradyzoites. Polymerase chain reaction (PCR) was employed to detect the parasite in the milk. Transmission to the offspring was verified by indirect immunofluorescence antibody test (IFAT), modified agglutination test (MAT), bioassay tests and PCR. Rat milk samples were PCR-positive, pups were serum-reactive to T. gondii and tissue samples also presented positive DNA results through PCR.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Toxoplasma gondii; Parasite in milk; Female rats; PCR; Bioassay.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000200016
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Insects as biological models to assay spider and scorpion venom toxicity J. Venom. Anim. Toxins incl. Trop. Dis.
Manzoli-Palma,M. F.; Gobbi,N.; Palma,M. S..
This study was undertaken to develop an experimental protocol using insects as biological models to assay venom toxicity of the following spiders Loxosceles gaucho, Phoneutria nigriventer, Nephilengys cruentata and Tityus serrulatus scorpion. Three different insect species were bioassayed: Apis mellifera (Hymenoptera), Grillus assimilis (Orthoptera), and Diatraea saccharalis (Lepidoptera). Venoms were injected into the hemocele of insects with a microsyringe at concentrations that caused dose/weight-dependent effects; doses causing either paralysis (ED50) or death (LD50) were recorded for each venom and insect test-species. T. serrulatus and L. gaucho venoms were lethal to all tested species, while P. nigriventer venom caused paralysis and death, and N....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bioassay; Venom; Insect model; Toxicity; Apis mellifera.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992003000200004
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Scorpion envenomation in pygmies from Democratic Republic of Congo, the example of Pelenge Center, Lomela, DRC J. Venom. Anim. Toxins incl. Trop. Dis.
Biezakala Mudiandambu,E; Odio Wobin,T; Kabele Ngiefu,C; Tati Kinkela,R; Mbanzulu Makola,K.
In a survey among the pygmies of central Democratic Republic of Congo, the incidence of scorpion stings seemed very high with a severity greater than expected. Species responsible were not identified. Specific studies are needed to clarify the risk emerging in the equatorial African forest.
Tipo: Info:eu-repo/semantics/other Palavras-chave: Arothron hispidus; Tetrodotoxin; Bioassay; ATPase and AchE enzymatic activities; Anticancer activity.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000400015
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Early-Stage Bioassay for Monitoring Radioactive Contamination in Living Livestock OAK
YAMAGUCHI, Toshiro; SAWANO, Kaita; KISHIMOTO, Miori; FURUHAMA, Kazuhisa; YAMADA, Kazutaka; 山田, 一孝.
Soil samples from the ground surface and feces and blood from a mixed-breed male pig were collected on April 10, 2011 at a farm within 20 km of the Fukushima Daiichi nuclear power plant. The radioactivity of each sample was measured using a Ge semiconductor detector. Despite the fact that the pig had been fed non-contaminated imported feed, 131I, 134Cs and 137Cs were detected in the feces, and 134Cs and 137Cs were detected in the blood clots. Because it is considerably difficult to measure radioactive contamination in the edible muscle of living livestock, bioassays are an option for the screening of radioactive contamination in living livestock to ensure food safety.
Palavras-chave: Bioassay; Cesium; Fukushima; Iodine; Radioactive contamination.
Ano: 2012 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3893
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Active biological materials inhibiting tumor initiation extracted from marine algae OceanDocs
Mostafa, M.H.; Ibrahim, A.M.M.; El-Masry, M.H.; El-Naggar, M.A..
Ethanol extraction of seventeen algal species from 35 different samples collected from the coastal area of Abou-Qir, Egypt, South East of the Mediterranean Sea Coast, was conducted. The aim was to obtain natural products from the algal extracts which have significant biologically active algal compounds. The collected algae showed a wide variation in their occurrence during the period of collection. Thirty five extracts representing different seasonal growths of 17 marine algal species were tested for antitumorigenic activity. Tests performed were the toxicity bioassay using Brine shrimp Artemia salina and the anti-tumor bioassay against Agrobacterium tumefaciens on potato discs. Codium tomentosum (winter); Jaina rubens (summer) and Padina pavonia (winter)...
Tipo: Journal Contribution Palavras-chave: Anti-tumor agent; Bioactive material; Bioassay; Marine algae; Natural product compound Biological properties Algae Marine environment http://aims.fao.org/aos/agrovoc/c_4610.
Ano: 2005 URL: http://hdl.handle.net/1834/1303
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